Development of an immunoanalytical method for the detection of β- and γ- crystalline and anti-crystallin antibodies. A molecular biomarker for cataract

Indian Journal of Ophthalmology

Volumn 50, Issue 1, 2002, Pages 41-48

  Nayak, Sujatha ^a   Sashidhar, R B ^a   Seetharam Bhat, K ^b  

^a OSMANIA UNIVERSITY   (India)

^b NATIONAL INSTITUTE OF NUTRITION   (India)

Author keywords

crystallins; crystallins; Anti crystallin antibody; Cataract

Indexed keywords

AUTOANTIBODY; BIOLOGICAL MARKER; CRYSTALLIN;

ANIMAL; ANIMAL MODEL; ARTICLE; BLOOD; CATARACT; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; ISOLATION AND PURIFICATION; LENS; MALE; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; RABBIT; RAT; WESTERN BLOTTING; WISTAR RAT;

ANIMALS; AUTOANTIBODIES; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CATARACT; CRYSTALLINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; LENS, CRYSTALLINE; MALE; MODELS, ANIMAL; RABBITS; RATS; RATS, WISTAR;

EID: 0036491096     PISSN: 03014738     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Thomas R, Muliyil J. The incidence of cataract in India is an overestimate. The National Med J of India 1998;11:182-84.
2. Dandona L, Dandona R, Naduvilath TJ, McCarty CA, Nanda A, Srinivas M, et al. Is current eye-care-policy focus almost exclusively on cataract adequate to deal with blindness in India? The Lancet 1998;351:1312-16.
3. Balasubramanian D, Bansal AK, Basti, S, Bhat KS, Murthy JS, Rao CM. The biology of cataract. Indian J Ophthalmol 1993;41:153-71.
4. Schaumberg DA, Glynn RJ, Christen WG, Hankinson SE, Hennekens CH. Relations of body fat distribution and height with cataract in men. Am J Clin Nutr 2000;72:1495-502.
5. Merck KB, de Haard-Hoekman WA, Cruysberg JRM, Bloemendal H, de Jong WW. Characterization of anticrystallin autoantibodies in patients with cataract. Mol Biol Rep 1993;17:93-99.
6. Ibaraki N, Lin L-R, Dang L, Reddy VN, Singh DP, Sueno T, et al. Anti-b-crystallin antibodies (mouse) or sera from humans with age-related cataract are cytotoxic for lens epithelial cells in culture. Exp Eye Res 1997;64:229-38.
7. Sueno T, Inoue E, Singh DP, Awata T, Chylack LT Jr, Shinohara T. Oral administration of lens homogenate suppresses antibody production in mice injected with β-crystallin emulsified in CFA. Exp Eye Res 1997;64:379-85.
8. Chamberlain CG, McAvoy JW. Fiber differentiation and polarity in the mammalian lens. A key role for FGF. Prog Ret Eye Res 1997;16:443-78.
9. Kannabiran C, Balasubramanian D. Molecular genetics of cataract. Indian J Ophthalmol 2000;48:5-13.
10. Chylack LT Jr, Singh DP, Kikuchi T, Sueno T, Wolfe J, Shinohara T. Human sera obtained from patients with cataract killed lens epithelial cells in culture. Invest Ophthalmol Vis Sci [Suppl] 1996;37/3:S1142.
11. Singh DP, Guru SC, Kikuchi T, Abe T, Shinohara T. Auto-antibodies against β-crystallins induce lens epithelial cell damage and cataract formation in mice. J Immunol 1995;155:993-99.
12. Niederkorn JY, Steilein JW. Induction of anterior chamber-associated immune deviation (ACIAD) by allogenic intraocular tumors does not require splenic metastases. J Immunol 1982;128:2470-74.
13. Fearon DT, Wong WW. Complement ligand-receptor interactions that mediated biological responses. Ann Rev Immunol 1983;1:243-71.
14. Bhat KS. Nutritional factors and cataract. In : Bamji MS, Rao NP, Reddy V, editors. Textbook of Human Nutrition. New Delhi; Oxford & IBH Publishing Co Pvt Ltd. 1996. pp 391-400.
15. Rao PV, Bhat KS. Effect of riboflavin deficiency on sorbitol pathway in rat lens. Nutr Res 1989;9:1143-49.
16. Bhat KS. Alterations in the lenticular proteins of rats on riboflavin deficient diet. Curr Eye Res 1983;2:829-34.
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
18. 4. Nature 1970;227:680-85.
19. Harlow E, Lane D. Antibodies: A laboratory Manual. New York: Cold Spring Harbor Laboratory; 1988. p 298-303.
20. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 1979;76:4350-54.
21. Chiou S-H, Chylack LT Jr, Tung WH, Bunn HF. Nonenzymatic glycosylation of bovine lens crystallins: Effect of aging. J Biol Chem 1981; 256: 5176-80
22. Angunawela II. The role of autoimmune phenomena in the pathogenesis of cataract. Immunology 1987;61:363-68.
23. Angunawela II. The effect of immunosuppression on the development of experimental cataract. Immunology 1988; 64:69-72.
24. th March. Amsterdam: Elsevier Science Publishers, 1990. pp 391-94.
25. Patel M, Shine B, Murray PI. Antilens antibodies in cataract and inflammatory eye disease: An evolution of a New Technique. Int Ophthalmol 1990;14:97-100.
26. Nissen SH, Andersen P, Andersen HMK. Antibodies to lens antigens in cataract and after cataract surgery. Br J Ophthalmol 1981;65:63-66.
27. Tanemoto K, Sueno T, Obazawa H, Shinohara T, Akatsuka A. Rat lens epithelial cell damage and cataract formation induced by immunological response to bovine lens membrane protein. Nippon-Ganka-Gakkai-Zasshi 1999;103:713-21.
28. Nakayama H, Mitsuhashi T, Kuwajima S, Aoki S, Kuroda Y, Itoh T, Nakagawa S. Immunochemical detection of advanced glycation end products in lens crystallins from strptozotocin - induced diabetic rat. Dibabetes 1993; 42:345-50.
29. John A, Bhat KS. Effect of riboflavin deficiency on low tryptophan induced cataract in male rats. Nutr Res 1992;12:1373-81.