Molecular cloning of the gene encoding thermostable endo-1,5-α-L-arabinase of bacillus thermodenitrificans TS-3 and its expression in bacillus subtilis

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 2, 2002, Pages 430-433

  Takao, Makoto ^a   Yamaguchi, Asako ^a   Yoshikawa, Kentaro ^a   Terashita, Takao ^a   Sakai, Takuo ^a  

^a KINKI UNIVERSITY   (Japan)

Author keywords

Bacillus; Endo 1,5 L arabinase; Gene cloning; Thermophile; Thermostable enzyme

Indexed keywords

ARABINAN ENDO 1,5 ALPHA L ARABINOSIDASE; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; GLYCOSIDASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

ASPERGILLUS; ASPERGILLUS NIGER; BACILLUS SUBTILIS; GEOBACILLUS THERMODENITRIFICANS; PROKARYOTA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0036479367     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.430     Document Type: Article

References (26)

1. Tanaka, M., Abe, A., and Uchida, T., Substrate specificity of alpha-L-arabinofuranosidase from plant Scopolia japonica calluses and a suggestion with reference to the structure of beet araban. Biochim. Biophys. Acta, 658, 377-386 (1981).
2. Sakamoto, T. and Sakai, T., Protopectinase-T: a rhamnogalacturonase able to solubilize protopectin from sugar beet. Carbohydr. Res., 259, 77-91 (1994).
3. Sakamoto, T. and Sakai, T., Analysis of structure of sugar-beet pectin by enzymatic methods. Phytochemistry, 39, 821-823 (1995).
4. Sakai, T. and Sakamoto, T., Purification and some properties of a protopectin-solubilizing enzyme that has potent activity on sugar beet protopectin. Agric. Biol. Chem., 54, 879-889 (1990).
5. Sakamoto, T., Yoshinaga, J., Shogaki, T., and Sakai, T., Studies on protopectinase-C mode of action: analysis of the chemical structure of the specific substrate in sugar beet protopectin and characterization of the enzyme activity. Biosci. Biotechnol. Biochem., 57, 1832-1837 (1993).
6. Voragen, A. G. J., Rombouts, F. M., Searle-van Leeuwen, M. F., Schols, H. A., and Pilnik, W., The degradation of arabinans by endo-arabinanase and arabinofuranosidases purified from Aspergillus niger. Food Hydrocolloids, 1, 423-437 (1987).
7. Kaji, A. and Saheki, T., Endo-arabinanase from Bacillus subtilis F-11. Biochim. Biophys. Acta, 410, 354-360 (1975).
8. Weinstein, L. and Albersheim, P., Structure of plant cell walls. Plant Physiol., 63, 425-432 (1979).
9. Yoshihara, O. and Kaji, A., An endo-1,5-a-L-arabinase, which can disintegrate potato tissue. Agric. Biol. Chem., 47, 1935-1940 (1983).
10. Mckie, V. A., Black, G. W., Millward-Sadler, S. J., Hazlewood, G. P., Laurie, J. I., and Gilbert, H. J., Arabinanase A from Pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo-mode of action. Biochem. J., 323, 547-555 (1997).
11. Ramon, D., V. D Veen P. and Visser, J., Arabinan degrading enzymes from Aspergillus nidulans: Induction and purification. FEMS Microbiol. Lett., 113, 15-22 (1993).
12. Flipphi, M. J. A., Panneman, H., V. D Veen P., Visser, J., and Graaff, L. H. de, Molecular cloning, expression and structure of the endo-1,5-alpha-L-arabinase gene of Aspergillus niger. Appl. Microbiol. Biotechnol., 40, 318-326 (1993).
13. Sakamoto, T., Yamada, M., Kawasaki, H., and Sakai, T., Molecular cloning and nucleotide sequence of an endo-1,5-a-L-arabinase gene from Bacillus subtilis. Eur. J. Biochem., 245, 708-714 (1997).
14. Moran, C. P. Jr., Lang, N., LeGrice, S. F. J., Lee, G., Stephens, M., Sonenshein, A. L., Pero, J., and Losick, R., Nucleotide sequences that signal the initiation of transcription and translation in Bacillus sub-tilis. Mol. Gen. Genet., 186, 339-346 (1982).
15. Henrissat, B. and Barioch, A., Updating the sequence-based classification of glycosyl hydrolases. Biochem. J., 316, 695-696 (1996).
16. Coutinho, P. M. and Henrissat, B. Carbohydorate-Active Enzymes Server at URL: http://afmb.cnrs-mrs.fr/'pedro/CAZY/db.html (2001)
17. Utt, E. A., Eddy, C. K., Keshav, K. F., and Ingram, L. O., Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabino-furanosidase activities. Appl. Environ. Microbiol., 57, 1227-1234 (1991).
18. Moriyama, H., Fukusaki, E., Cabrera Crespo, J., Shinmyo, A., and Okada, H., Structure and expression of genes coding for xylan-degrading enzymes of Bacillus pumilus. Eur. J. Biochem., 166, 539-545 (1987).
19. Gosalbes, M. J., Perez-Gonzalez, J. A., Gonzalez, R., and Navarro, A., Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase. J. Bacteriol., 173, 7705-7710 (1991).
20. Takao, M., Nakaniwa, T., Yoshikawa, K., Terashita, T., and Sakai, T., Purification and characterization of thermostable pectate lyase with protopectinase activity from thermophilic Bacillus sp. TS 47. Biosci. Biotechnol. Biochem., 64, 2360-2367 (2000).
21. Takao, M., Nakaniwa, T., Yoshikawa, K., Terashita, T., and Sakai, T., Molecular cloning, DNA sequence, and expression of the gene encoding for thermostable pectate lyase of thermophilic Bacillus sp. TS 47. Biosci. Biotechnol. Biochem., 65, 322-329 (2001).
22. Nasser, W., Chalet, F., and Robert-Baudouy, J., Purification and characterization of extracellular pec-tate lyase from Bacillus subtilis. Biochimie., 72, 689-695 (1990).
23. Nasser, W., Awade, A. C., Reverchon, S., and Robert-Baudouy, J., Pectate lyase from Bacillus subtilis: molecular characterization of the gene, and properties of the cloned enzyme. FEBS Lett., 335, 319-326 (1993).
24. Ehrlich, S. D., Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc. Natl. Acad. Sci. USA, 74, 1680-1682 (1977).
25. Gryczan, T. J., Contente, S. and Dubnau, D., Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol., 134, 318-329 (1978).
26. Somogyi, M., Note on sugar determination. J. Biol. Chem., 195, 19-23 (1952).