Production of brain-derived neurotrophic factor in escherichia coli by coexpression of dsb proteins

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 2, 2002, Pages 344-350

  Hoshino, Kyoko ^a   Eda, Akiko ^a   Kurokawa, Yoichi ^b,c   Shimizu, Norio ^a  

^a TOYO UNIVERSITY   (Japan)

^b Kyoto University   (Japan)

^c FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

BDNF; Cysteine oxidoreductase; Dsb proteins; Escherichia coli; Periplasm

Indexed keywords

BRAIN DERIVED NEUROTROPHIC FACTOR; ESCHERICHIA COLI PROTEIN;

ARTICLE; BIOSYNTHESIS; CULTURE MEDIUM; ESCHERICHIA COLI; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

BLOTTING, WESTERN; BRAIN-DERIVED NEUROTROPHIC FACTOR; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS;

ESCHERICHIA COLI;

EID: 0036479359     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.344     Document Type: Article

References (16)

1. Lindsay, R. M., Brain-derived neurotrophic factor: an NGF-related neurotrophin. In “Neurotrophic Factors”, eds. Loughlin, S. E. and Fallon, J. H., Academic Press, Inc., San Diego, pp. 257-284 (1993).
2. Negro, A., Corsa, V., Moretto, C., Skaper, S. D., and Callegaro, L., Synthesis and purification of biologically active rat brain-derived neurotrophic factor from Escherichia coli. Biochem. Biophys. Res. Commun, 186, 1553-1559 (1992).
3. Fukuzono, S., Fujimori, K., and Shimizu, N., Production of biologically active mature brain derived neurotrophic factor in Escherichia coli. Biosci. Biotechnol. Biochem., 59, 1727-1731 (1995).
4. Shimizu, N., Fukuzono, S., Fujimori, K., Park, K., Takeshita, T., and Ishibashi, T., Biological activity of brain-derived neurotrophic factor with mismatched disulfide linkages produced by Escherichia coli. Biosci. Biotechnol. Biochem., 60, 971-974 (1996).
5. Missiakas, D. and Raina, S., Protein folding in the bacterial periplasm. J. Bacteriol., 179, 2465-2471 (1997).
6. Kobayashi, T., Kishigami, S., Sone, M., Inokuchi, H., Mogi, T., and Ito, K., Respiratory chain is required to maintain oxidized states of the DsbA-DsbB disulfide bond formation system in aerobically growing Escherichia coli cells. Proc. Natl. Acad. Sci. USA, 94, 11857-11862 (1997).
7. Debarbieux, L. and Beckwith, J., The reductive enzyme thioredoxin 1 acts as an oxidant when it is exported to the Escherichia coli periplasm. Proc. Natl. Acad. Sci. USA, 95, 10751-10756 (1998).
8. Joly, J. C., Leung, W. S., and Swartz, J. R., Overexpression of Escherichia coli oxidoreductases increases recombinant insulin-like growth factor-I accumulation. Proc. Natl. Acad. Sci. USA, 95, 2773-2777 (1998).
9. Qiu, J., Swartz, J. R., and Georgiou, G., Expression of active human tissue-type plasminogen activator in Escherichia coli. Appl. Environ. Microbiol., 64, 4891-4896 (1998).
10. Kurokawa, Y., Yanagi, H., and Yura, T., Overexpression of protein disulfide isomerase DsbC stabilizes multiple-disulfide-bonded recombinant protein produced and transported to the periplasm in Escherichia coli. Appl. Environ. Microbiol., 66, 3960-3965 (2000).
11. Kondo, A., Kohda, J., Endo, Y., Shiromizu, T., Kurokawa, Y., Nishihara, K., Yanagi, H., Yura, T., and Fukuda, H., Improvement of productivity of active horseradish peroxidase in Escherichia coli by coexpression of Dsb proteins. J. Biosci. Bioeng., 90, 600-606 (2000).
12. Kurokawa, Y., Yanagi, H., and Yura, T., Overproduction of bacterial protein disulfide isomerase (DsbC) and its modulator (DsbD) markedly enhances periplasmic production of human nerve growth factor in Escherichia coli. J. Biol. Chem., 276, 14393-14399 (2001).
13. Fujimori, K., Fukuzono, S., Kotomura, N., Kuno, N., and Shimizu, N., Overproduction of biologically-active human nerve growth factor in Escherichia coli. Biosci. Biotechnol. Biochem., 56, 1985-1990 (1992).
14. Nishihara, K., Kanemori, M., Yanagi, H., and Yura, T., Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli. Appl. Environ. Microbiol., 66, 884-889 (2000).
15. Ostermeier, M., Sutter, K. D., and Georgiou, G., Eukaryotic protein disulfide isomerase complements Escherichia coli dsbA mutants and increases the yield of a heterologous secreted protein with disulfide bonds. J. Biol. Chem., 271, 10616-10622 (1996).
16. Zhan, X., Schwaller, M., Gilbert, H. F., and Georgiou, G., Facilitating the formation of disulfide bonds in the Escherichia coli periplasm via coexpression of yeast protein disulfide isomerase. Biotechnol. Prog., 15, 1033-1038 (1999).