Overexpression, purification and biochemical characterization of a class a high-molecular-mass penicillin-binding protein (PBP), PBP1* and its soluble derivative from Mycobacterium tuberculosis

Biochemical Journal

Volumn 361, Issue 3, 2002, Pages 635-639

  Bhakta, Sanjib ^a   Basu, Joyoti ^a  

^a BOSE INSTITUTE   (India)

Author keywords

lactam; Cell wall biosynthesis; Transpeptidase

Indexed keywords

AMINO ACIDS; DEGRADATION; ESCHERICHIA COLI; GENES; MOLECULAR WEIGHT;

SPHEROPLASTS;

PROTEINS;

AMOXICILLIN; AMPICILLIN; BETA LACTAM; CEFOTAXIME; CEFOXITIN; CEFTRIAXONE; CEFUROXIME; CHLORIDE; GAMMA GLUTAMYLTRANSFERASE; GENE PRODUCT; GLUTAMINE; GLYCINE; GUANIDINE; PENICILLIN BINDING PROTEIN; PENICILLIN G; PROTEIN A; PROTEINASE K; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENOME; BACTERIAL MEMBRANE; BINDING AFFINITY; CELL INCLUSION; CONTROLLED STUDY; COSMID; CYTOPLASM; CYTOSOL; ESCHERICHIA COLI; GENE OVEREXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCOBACTERIUM LEPRAE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; SPHEROPLAST;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BLOTTING, WESTERN; CARRIER PROTEINS; CELL MEMBRANE; CYTOSOL; ENDOPEPTIDASE K; ESCHERICHIA COLI; GENE DELETION; GLUTAMINE; GLYCINE; HEXOSYLTRANSFERASES; KINETICS; MOLECULAR SEQUENCE DATA; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; MYCOBACTERIUM TUBERCULOSIS; OPEN READING FRAMES; PENICILLIN-BINDING PROTEINS; PENICILLINS; PEPTIDYL TRANSFERASES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM LEPRAE; MYCOBACTERIUM TUBERCULOSIS; UNCULTURED ACTINOMYCETE;

EID: 0036471217     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3610635     Document Type: Article

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