Complete sequencing of anti-vancomycin fab fragment by liquid chromatography-electrospray ion trap mass spectrometry with a combination of database searching and manual interpretation of the MS/MS spectra

Journal of Immunological Methods

Volumn 260, Issue 1-2, 2002, Pages 235-249

  Adamczyk, Maciej ^a   Gebler, John C ^a   Wu, Jiang ^a   Yu, Zhiguang ^a  

^a ABBOTT DIAGNOSTICS   (United States)

Author keywords

Anti vancomycin antibody; Database searching; Electrospray mass spectrometry; Fab; Sequencing

Indexed keywords

GLUTAMINE; IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY; PYROGLUTAMIC ACID;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; DATA BASE; INFORMATION RETRIEVAL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTON TRANSPORT; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, LIQUID; DATABASES, PROTEIN; IMMUNOGLOBULIN FAB FRAGMENTS; MASS SPECTROMETRY; MICE; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS; VANCOMYCIN;

EID: 0036470424     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00552-X     Document Type: Article

References (41)

1. Electrospray mass spectrometry of α and β chains of selected hemoglobins and their TNBA and TNB conjugates
2. Immunoassay reagents for thyroid testing: 2. Binding properties and energetic parameters of a T4 monoclonal antibody and its Fab fragment with a library of thyroxine analog biosensors using surface plasmon resonance
3. The use of electrospray ionization mass spectrometry to distinguish the lot-to-lot heterogeneity of an antigen-specific monoclonal antibody from a specific cellular clone
4. Charge derivatization of peptides to simplify their sequencing with an ion trap mass spectrometer
5. A simple method to identify cysteine residues by isotopic labeling and ion trap mass spectrometry
6. Sequencing of anti-thyroxine monoclonal antibody Fab fragment by ion trap mass spectrometry
7. Characterization of mouse switch variant antibodies by matrix-assisted laser desorption ionization mass spectrometry and electrospray ionization mass spectrometry
8. Mass spectrometry of the humanized monoclonal antibody CAMPATH 1H
9. Rapid characterization of chemically modified proteins by electrospray mass spectrometry
10. Monitoring papain digestion of a monoclonal antibody by electrospray ionization mass spectrometry
11. C-terminal sequence analysis of peptides and proteins using carboxypeptidases and mass spectrometry after derivatization of Lys and Cys residues
12. Electrospray ionization mass spectrometry of recombinantly engineered antibody fragments
13. Analysis of antibodies and other large glycoproteins in the mass range of 150 000-200 000 Da by electrospray ionization mass spectrometry
14. High sensitivity identification of proteins by electrospray ionization tandem mass spectrometry: Initial comparison between an ion trap mass spectrometer and a triple quadrupole mass spectrometer
15. 2 fragment of a murine monoclonal antibody using capillary isoelectric focusing and electrospray ionization mass spectrometry
16. Complete and rapid peptide and glycopeptide mapping of mouse monoclonal antibody by LC/MS/MS using ion trap mass spectrometry
17. A facile picomole-scale method for charge derivatization of peptides for sequence analysis by mass spectrometry
18. Protein sequencing by tandem mass spectrometry
19. Application of electrospray mass spectrometry (ES-MS) for the analysis of monoclonal antibody Fc subunits
20. Characterization of humanized anti-TAC, an antibody directed against the interleukin 2 receptor, using electrospray ionization mass spectrometry by direct infusion, LC/MS, and MS/MS
21. Accessing the Kabat antibody sequence database by computer
22. The interpretation of collision-induced dissociation tandem mass spectra of peptides
23. On the fragmentation of monoclonal IgG1, IgG2a, and IgG2b from BALB/c mice
24. Chapter 14: Preparation and purification of active fragments from mouse monoclonal antibodies
25. Monoclonal antibodies: Purification, fragmentation and application to structural and functional studies of class I MHC antigens
26. Structural determination of a monoclonal Fab fragment specific for histidine-containing protein of the phosphoenolpyruvate: Sugar phosphotransferase system of Escherichia Coli
27. An integrated strategy for structural characterization of the protein and carbohydrate components of monoclonal antibodies: Application to anti-respiratory syncytial virus MAb
28. Evaluation of charge derivatization of a proteolytic protein digest for improved mass spectrometric analysis: De novo sequencing by matrix-assisted laser desorption/ionization post-source decay mass spectrometry
29. Peptide sequencing by mass spectrometry for homology searches and cloning of genes
30. Papain hydrolysis products in four M-IgG subclasses
31. MALDI-MS for C-terminal sequence determination of peptides and proteins degraded by carboxypeptidase Y and P
32. Probing the proline effect in CID of protonated peptides
33. Proteolytic fragmentation with high specificity of mouse immunoglobulin G: Mapping of proteolytic cleavage sites in the hinge region
34. Database searching using mass spectrometry data
35. Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database
36. Direct analysis of protein mixtures by tandem mass spectrometry
37. Automated protein identification using microcolumn liquid chromatography-tandem mass spectrometry
38. Comparison of four methods to generate immunoreactive fragments of a murine monoclonal antibody OC859 against human ovarian epithelial cancer antigen