Reconstructed skin kits: Reproducibility of cutaneous irritancy testing

Skin Pharmacology and Applied Skin Physiology

Volumn 15, Issue SUPPL. 1, 2002, Pages 74-91

  Faller, C ^a   Bracher, M ^a  

^a Wella Cosmital SA   (Switzerland)

Author keywords

Cutaneous irritancy; In vitro testing; Reconstructed human epidermis; Reproducibility

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; CYTOKINE; DODECYL SULFATE SODIUM; ENZYME; INTERLEUKIN 1ALPHA; LACTATE DEHYDROGENASE;

ARTICLE; BIOLOGICAL MODEL; CELL SECRETION; CYTOKINE RELEASE; CYTOSOL; CYTOTOXICITY; EPIDERMIS; IN VITRO STUDY; INFLAMMATION; MEDIATOR; PRIORITY JOURNAL; PROTEIN ANALYSIS; REPRODUCIBILITY; SKIN; SKIN IRRITATION; TIME; TISSUE CULTURE; TOXICITY TESTING; ARTIFICIAL SKIN; COMPARATIVE STUDY; HUMAN; METHODOLOGY; STANDARD; STATISTICS;

HUMANS; SKIN IRRITANCY TESTS; SKIN, ARTIFICIAL;

EID: 0036460003     PISSN: 14222868     EISSN: None     Source Type: Journal    
DOI: 10.1159/000066678     Document Type: Article

References (24)

1. Prunieras M, Regnier M, Schloetterer M: A new method to culture human epidermal cells on allogeneic or xenogeneic dermis: Preparation of recombined grafts. Ann Chir Plast 1979;24:357-362.
2. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T: Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science 1981;211:1051-1054.
3. Noser FK, Limat A: Organotypic culture of outer root sheath cells from human hair follicles using a new culture device. In Vitro Cell Dev Biol 1987;23:541-545.
4. Rosdy M, Clauss LC: Terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface. J Invest Dermatol 1990;95:409-414.
5. Tinois E, Tiollier J, Gaucherand M, Dumas H, Tardy M, Thivolet J: In vitro and post-transplantation differentiation of human keratinocytes grown on the human type IV collagen film of a bilayered dermal substitute. Exp Cell Res 1991;193:310-319.
6. Cannon CL, Neal PJ, Southee JA, Kubilus J, Klausner M: New epidermal model for dermal irritancy testing. Toxicol In Vitro 1994;8:889-891.
7. Wahlen E, Donnelly TA, Naughton G, Rheins LA: The development of three-dimensional in vitro human tissue models. Hum Exp Toxicol 1994;13:853-859.
8. Prunieras M: Skin and epidermal equivalents: A review; in Rougier A, Goldberg A, Maibach HI (eds): In Vitro Skin Toxicology Irritation, Phototoxicity, Sensibilization. New York, Mary Ann Liebert, 1994, pp 97-105.
9. Limat A, Hunziker T: Epidermal equivalents generated from cultured outer root sheath cells. In Vitro Toxicology 1997;10:33-38.
10. Bell E, Parenteau N, Gay R, Nolte C, Kemp P, Bilbo P, Ekstein B, Johnson E: The living skin equivalent: Its manufacture, its organotypic properties and its responses to irritants. Toxicol In Vitro 1991;5:591-596.
11. Gay R, Swiderek M, Nelson D, Ernesti A: The living skin equivalent as a model in vitro for ranking the toxic potential of dermal irritants. Toxicol In Vitro 1992;6:303-315.
12. Slivka SR, Zeigler F: Use of an in vitro skin model for determining epidermal and dermal contributions to irritant responses. J Toxicol Cutan Ocular Toxicol 1993;12:49-57.
13. Ponec M, Kempenaar J: Use of human skin recombinants as an in vitro model for testing the irritation potential of cutaneous irritants. Skin Pharmacol 1995;8:49-59.
14. Roguet R, Cohen C, Dossou KG, Rougier A: Episkin, a reconstituted human epidermis for assessing in vitro the irritancy of topically applied compounds. Toxicol In Vitro 1994;8:283-291.
15. Roguet R, Régnier M, Cohen C, Dossou KG, Rougier A: The use of in vitro reconstituted human skin in dermotoxicity testing. Toxicol In Vitro 1994;8:635-639.
16. Ponec M: The use of in vitro skin recombinants to evaluate cutaneous toxicity; in Rougier A, Goldberg A, Maibach HI (eds): In Vitro Skin Toxicology Irritation, Phototoxicity, Sensibilization. New York, Mary Ann Liebert, 1994, pp 107-116.
17. Roguet R: Use of skin cell cultures for in vitro assessment of corrosion and cutaneous irritancy. Cell Biol Toxicol 1999;15:63-75.
18. Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytoxicity assays. J Immunol Methods 1983;65:55-63.
19. Tenenhaus M: Groupe HEC, 78351, Jouy-en-Josas, Utilisation Varcomp et Mixed.
20. SAS/STAT®, User's Guide Volume 2, GLM-VARCOMP, Version 6, Fourth Edition.
21. XXXIes Journées de Statistique 17-21 mai 1999, Grenoble, France, C. Agut: Modèle mixte - Une application aux essais inter-laboratoires.
22. Saporta G: Probabilités, Analyse des Données et Statistique. Paris, Editions Technip, 1990.
23. Boelsma E, Gibbs S, Faller C, Ponec M: Characterization and comparison of reconstructed skin models: Morphological and immunohistochemical evaluation. Acta Derm Venereol 2000;80:82-88.
24. Efron B: Le Bootstrap et Ses Applications. Discrimination et Régression. Saint-Mandé, CISIA, 1995.