Technical considerations for RNA-based stable isotope probing: An approach to associating microbial diversity with microbial community function

Rapid Communications in Mass Spectrometry

Volumn 16, Issue 23, 2002, Pages 2179-2183

  Manefield, Mike ^a   Whiteley, Andrew S ^a   Ostle, Nick ^a   Ineson, Philip ^b   Bailey, Mark J ^a  

^a CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

^b UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ATOMS; BIODEGRADATION; BIOMARKERS; BUOYANCY; CENTRIFUGATION; ELECTROPHORESIS; ISOTOPES; MASS SPECTROMETRY; POLYMERASE CHAIN REACTION;

BIOLOGICAL SIGNATURES; BUOYANT DENSITY; COMMUNITY FUNCTION; CURRENT LIMITATION; MICROBIAL COMMUNITIES; MICROBIAL DIVERSITY; MICROBIAL ECOLOGY; STABLE ISOTOPES; STABLE-ISOTOPE PROBING; TRACER COMPOUNDS;

RNA;

BACTERIAL RNA; BIOLOGICAL MARKER; CARBON; CARBON 13; ISOTOPE; PHENOL; RNA; TRACER; XENOBIOTIC AGENT;

AGAR GEL ELECTROPHORESIS; ATOM; BIODEGRADATION; CLASSIFICATION; CONFERENCE PAPER; DENATURING GRADIENT GEL ELECTROPHORESIS; DENSITY GRADIENT CENTRIFUGATION; ECOLOGY; GENETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MICROBIAL DIVERSITY; MICROBIOLOGY; MICROFLORA; MOLECULAR CLONING; MOLECULAR GENETICS; PSEUDOMONAS PUTIDA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PROBE;

EID: 0036452120     PISSN: 09514198     EISSN: None     Source Type: Journal    
DOI: 10.1002/rcm.782     Document Type: Conference Paper

References (9)

1. Gray ND, Head IM. Environ. Microbial. 2001; 3: 481.
2. Lee N, Nielsen PH, Andreasen KH, Juretschko S, Nielsen JL, Schleifer KH, Wagner M. Appl. Environ. Microbiol. 1999; 65: 1289.
3. Radajewski S, Ineson P, Parekh NR, Murrell JC. Nature 2000; 403: 646.
4. Boschker HTS, Nold SC, Wellsbury P, Bos D, de Graaf W, Pel R, Parkes RJ, Cappenberg T. Nature 1998; 392: 801.
5. Bull ID, Parekh NR, Hall GH, Ineson P, Evershed RP. Nature 2000; 405: 175.
6. Manefield M, Whiteley A, Ineson P, Bailey M. Appl. Env. Microbiol. (submitted).
7. Whiteley AS, Wiles S, Lilley AK, Philp J, Bailey MJ. J Microbiol. Methods 2000; 44: 79.
8. Rickwood D (ed). Centrifugation: A Practical Approach (2nd Edn). IRL Press: Oxford, 1984.
9. Muyzer G, Smalla K. Antonie Van Leeuwenhoek 1998; 73: 127.