High-yield method for isolation and culture of endothelial cells from rat coronary blood vessels suitable for analysis of intracellular calcium and nitric oxide biosynthetic pathways

Biological Procedures Online

Volumn 4, Issue 1, 2002, Pages 32-37

  Nistri, Silvia ^a   Mazzetti, Luca ^b   Failli, Paola ^b   Bani, Daniele ^a  

^a Section of Histology Enrico Allara   (Italy)

^b UNIVERSITY OF FLORENCE   (Italy)

Author keywords

Acetylated LDL; DAF 2 DA; Endothelial cells; Fura 2AM; Nitric oxide

Indexed keywords

CALCIUM; COLLAGENASE; FLUORESCENT DYE; HEPARIN; KETAMINE; LOW DENSITY LIPOPROTEIN; NITRIC OXIDE; VASOACTIVE AGENT; XYLAZINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOSYNTHESIS; CALCIUM CELL LEVEL; CELL CULTURE; CELL FUNCTION; CELL ISOLATION; CHEMICAL LABELING; CONTROLLED STUDY; CORONARY ARTERY BLOOD FLOW; CORONARY BLOOD VESSEL; DRUG RESPONSE; ENDOTHELIUM CELL; ENZYME LOCALIZATION; ISOLATION PROCEDURE; LIPID TRANSPORT; METHODOLOGY; NONHUMAN; RAT; REPRODUCIBILITY; THERMOREGULATION; UPREGULATION;

EID: 0036449468     PISSN: 14809222     EISSN: None     Source Type: Journal    
DOI: 10.1251/bpo31     Document Type: Article

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