Cysteine metabolism in periportal and perivenous hepatocytes: Perivenous cells have greater capacity for glutathione production and taurine synthesis but not for cysteine catabolism

Amino Acids

Volumn 23, Issue 4, 2002, Pages 453-458

  Bella, D L ^a   Hirschberger, L L ^a   Kwon, Y H ^a   Stipanuk, Martha H ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

Glutamylcysteine synthetase; Cystathionase; Cysteine dioxygenase; Cysteinesulfinate decarboxylase; Periportal hepatocytes; Perivenous hepatocytes

Indexed keywords

ASPARTATE AMINOTRANSFERASE; COLLAGENASE; CYSTATHIONINE GAMMA LYASE; CYSTEINE; CYSTEINE DIOXYGENASE; CYSTEINESULFINATE DECARBOXYLASE; DIGITONIN; ENZYME; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; TAURINE; UNCLASSIFIED DRUG; ADENOSINE TRIPHOSPHATE;

ANIMAL CELL; ARTICLE; CELL TYPE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME LOCALIZATION; INCUBATION TIME; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN METABOLISM; PROTEIN SYNTHESIS; RAT; ANIMAL; CYTOLOGY; ENZYMOLOGY; MALE; METABOLISM; SPRAGUE DAWLEY RAT;

ANIMALIA;

ADENOSINE TRIPHOSPHATE; ANIMALS; CYSTEINE; GLUTATHIONE; HEPATOCYTES; MALE; RATS; RATS, SPRAGUE-DAWLEY; TAURINE;

EID: 0036449448     PISSN: 09394451     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00726-002-0213-z     Document Type: Article

References (31)

1. Bagley PJ, Hirschberger LL, Stipanuk MH (1995) Evaluation and modification of an assay procedure for cysteine dioxygenase activity: High-performance liquid chromatography method for measurement of cysteine sulfinate and demonstration of physiological relevance of cysteine dioxygenase activity in cysteine catabolism. Anal Biochem 227: 40-48
2. Bagley PJ, Stipanuk MH (1994) The activities of rat hepatic cysteine dioxygenase and cysteinesulfinate decarboxylase are regulated in a reciprocal manner in response to dietary casein level. J Nutr 124: 2410-2421
3. Bagley PJ, Stipanuk MH (1995) Rats fed a low protein diet supplemented with sulfur amino acids have increased cysteine dioxygenase activity and increased taurine production in hepatocytes. J Nutr 125: 933-940
4. Bars RG, Bell DR, Elcombe CR, Oinoen T, Jalava T, Lindros KO (1992) Zone-specific inducibility of cytochrome P450 2B1/2 is retained in isolated perivenous hepatocytes. Biochem J 282: 635-638
5. Bella DL, Stipanuk MH (1995) Effects of protein, methionine, or chloride on acid-base balance and on cysteine catabolism. Am J Physiol 269: E910-E917
6. Bella DL, Hahn C, Stipanuk MH (1999a) Effects of nonsulfur and sulfur amino acids on the regulation of hepatic enzymes of cysteine metabolism. Am J Physiol 277: E144-E153
7. Bella DL, Hirschberger LL, Hosokawa Y, Stipanuk MH (1999b) Mechanisms involved in the regulation of key enzymes of cysteine metabolism in rat liver in vivo. Am J Physiol 276: E326-E335
8. Bergmeyer HU, Bernt E (1974) Glutamate-oxaloacetate transaminase. In: Bergmeyer HU (ed) Methods of Enzymatic Analysis, vol. 2. Academic Press, New York, pp 752-758
9. Berry MN, Friend DS (1969) High-yield preparation of isolated rat liver parenchymal cells: A biochemical and fine structural study. J Cell Biol 43: 506-520
10. Chen KS, Katz J (1985) Zonation of glycogen and glucose syntheses, but not glycolysis, in rat liver. Biochem J 255: 99-104
11. Daniels KM, Stipanuk MH (1982) The effect of dietary cysteine level on cysteine metabolism in rats. J Nutr 112: 2130-2141
12. Drake MR, De La Rosa J, Stipanuk MH (1987) Metabolism of cysteine in rat hepatocytes. Evidence for cysteinesulphinate-independent pathways. Biochem J 244: 279-286
13. Gebhardt R (1992) Metabolic zonation of the liver: Regulation and implications for liver function. Pharmacol Ther 53: 275-354
14. Gebhardt R, Mecke D (1983) Heterogeneous distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture. Embo J 2: 567-570
15. Kera Y, Penttila KE, Lindros KO (1988) Glutathione replenishment capacity is lower in isolated perivenous than in periportal hepatocytes. Biochem J 254: 411-417
16. Krebs HA, Cornell NW, Lund P, Hems R (1974) Isolation of liver cells as experimental material. In: Lindquist F, Tygstrup N (eds) Regulation of Hepatic Metabolism. Academic Press, New York, pp 726-750
17. Lamprecht W, Trautschold I (1974) ATP determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergmeyer HU (ed) Methods of Enzymatic Analysis, vol 4. Academic Press, New York, pp 2101-2110
18. Ohta J, Kwon YH, Stipanuk MH (2000) Cysteine dioxygenase and γ-glutamylcysteine synthetase activities in primary cultured hepatocytes respond to sulfur amino acid supplementation in a reciprocal manner. Amino Acids 19: 705-728
19. Parsons RB, Ramsden DB, Waring RH, Barber PC, Williams AC (1998) Hepatic localisation of rat cysteine dioxygenase. J Hepatol 29: 595-602
20. Pentilla KE (1990) Role of cysteine and taurine in regulating glutathione synthesis by periportal and perivenous hepatocytes. Biochem J 269: 659-664
21. Quistorff B (1985) Gluconeogensis in periportal and perivenous hepatocytes of rat liver, isolated by a new high-yield digitonin/collagenase perfusion technique. Biochem J 229: 221-226
22. Saiki H, Chan ET, Wong E, Yamamuro W, Ookhtens M, Kaplowitz N (1992) Zonal distribution of cysteine uptake in the perfused rat liver. J Biol Chem 267: 192-196
23. Shimada M, Koide T, Kuroda E, Tsuboyama N, Hosokawa Y, Watanabe M (1998) Expression and localization of cysteine dioxygenase mRNA in the liver, lung, and kidney of the rat. Amino Acids 15: 143-150
24. Smith MT, Loveridge N, Wills ED, Chayen J (1979) The distribution of glutathione in the rat liver lobule. Biochem J 182: 103-108
25. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85
26. Stipanuk MH (1979) Effect of excess dietary methionine on the catabolism of cysteine in rats. J Nutr 109: 2126-2139
27. Stipanuk MH, Coloso RM, Garcia RA, Banks MF (1992) Cysteine concentration regulates cysteine metabolism to glutathione, sulfate and taurine in rat hepatocytes. J Nutr 122: 420-427
28. Ugele B, Kempen HJ, Kempen JM, Gebhardt R, Meijer P, Burger HJ, Princen HM (1991) Heterogeneity of rat liver parenchyma in cholesterol 7 alpha-hydroxylase and bile acid synthesis. Biochem J 276: 73-77
29. Vorhaben JE, Wong L, Campbell JW (1973) Assay for glutamine synthetase activity. Biochem J 135: 893-896
30. Watford M, Smith EM (1990) Distribution of hepatic glutaminase activity and mRNA in perivenous and periportal rat hepatocytes. Biochem J 267: 265-267
31. Watford M, Smith EM, Erbelding EJ (1984) The regulation of phosphate-activated glutaminase activity and glutamine metabolism in the streptozotocin-diabetic rat. Biochem J 224: 207-214