Plant pyruvate-dependent gamma-aminobutyrate transaminase: Identification of an Arabidopsis cDNA and its expression in Escherichia coli

Canadian Journal of Botany

Volumn 80, Issue 9, 2002, Pages 933-941

  Van Cauwenberghe, Owen R ^a,b   Makhmoudova, Amina ^a   McLean, Michael D ^a   Clark, Shawn M ^a   Shelp, Barry J ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b ELI LILLY AND COMPANY   (United States)

Author keywords

Amino acceptor; Gamma aminobutyrate; Gamma aminobutyrate transaminase; Heterologous expression; Protein purification; Recombinant protein

Indexed keywords

AMINO ACIDS; AMPLIFICATION; DNA; ELECTROPHORESIS; ESCHERICHIA COLI; NITROGEN; POLYPEPTIDES; PROTEINS;

MITOCHONDRIAL ISOLATION;

PLANTS (BOTANY);

2 OXOGLUTARIC ACID; 3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; 4 AMINOBUTYRATE AMINOTRANSFERASE; 4 AMINOBUTYRIC ACID; COMPLEMENTARY DNA; PLANT EXTRACT; PYRIDOXAL 5 PHOSPHATE; SIGNAL PEPTIDE;

BACTERIUM;

AMINO ACID SEQUENCE; COMPUTER ANALYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; MITOCHONDRION; OPEN READING FRAME; PLANT LEAF; PROTEIN EXPRESSION;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS); EMBRYOPHYTA; ESCHERICHIA COLI; NICOTIANA; NICOTIANA TABACUM;

EID: 0036445217     PISSN: 00084026     EISSN: None     Source Type: Journal    
DOI: 10.1139/b02-087     Document Type: Article

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