Distribution of basement membrane pores in bronchus revealed by microscopy following epithelial removal

Journal of Structural Biology

Volumn 139, Issue 3, 2002, Pages 137-145

  Howat, William J ^a,b   Barabás, Tony ^a   Holmes, James A ^a   Holgate, Stephen T ^a   Lackie, Peter M ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

Cell trafficking; Confocal laser scanning microscopy; Nearest neighbour analysis; Scanning electron microscopy; Transmission electron microscopy

Indexed keywords

DITHIOTHREITOL; EDETIC ACID;

ARTICLE; BASAL CELL; BASEMENT MEMBRANE; BASEMENT MEMBRANE PORE; BRONCHUS; BRONCHUS MUCOSA; CELL INFILTRATION; COLUMNAR EPITHELIUM; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EPITHELIUM CELL; HUMAN; HUMAN TISSUE; INCUBATION TIME; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SIMULATION; SUBMUCOSA; TISSUE CHARACTERIZATION; TISSUE CULTURE; TISSUE PREPARATION;

BASEMENT MEMBRANE; BRONCHI; EPITHELIUM; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING;

BRONCHUS;

EID: 0036444252     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1047-8477(02)00589-0     Document Type: Article

References (13)

1. Beasley, R., Roche, W.R., Roberts, J.A., Holgate, S.T., 1989. Cellular events in the bronchi in mild asthma and after bronchial provocation. Am. Rev. Respir. Dis. 139, 806-817.
2. Evans, M.J., Plopper, C.G., 1988. The role of basal cells in adhesion of columnar epithelium to airway basement membrane. Am. Rev. Respir. Dis. 138, 481-483.
3. Gunzer, M., Kampgen, E., Brocker, E.B., Zanker, K.S., Friedl, P., 1997. Migration of dendritic cells in 3D-collagen lattices. Visualisation of dynamic interactions with the substratum and the distribution of surface structures via a novel confocal reflection imaging technique. Adv. Exp. Med. Biol. 417, 97-103.
4. Heck, L.W., Blackburn, W.D., Irwin, M.H., Abrahamson, D.R., 1990. Degradation of basement membrane laminin by human neutrophil elastase and cathepsin-G. Am. J. Pathol. 136, 1267-1274.
5. Hogan, B.L.M., 1999. Morphogenesis. Cell 96, 225-233.
6. Howat, W.J., Holmes, J.A., Holgate, S.T., Lackie, P.M., 2001. Basement membrane pores in human bronchial epithelium: A conduit for infiltrating cells? Am. J. Pathol. 158, 673-680.
7. Jones, K.H., Senft, J.A., 1985. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide. J. Histochem. Cytochem. 33, 77-79.
8. Kobayashi, Y., Matsumoto-Kobayashi, M., Kotani, M., Makino, T., 1999. Possible involvement of matrix metalloproteinase-9 in Langerhans cell migration and maturation. J. Immunol. 163, 5989-5993.
9. Leppert, D., Waubant, E., Galardy, R., Bunnett, N.W., Hauser, S.L., 1995. T-cell gelatinases mediate basement membrane transmigration in vitro. J. Immunol. 154, 4379-4389.
10. Mahida, Y.R., Galvin, A.M., Gray, T., Makh, S., McAlindon, M.E., Sewell, H.F., 1997. Migration of human intestinal lamina propria lymphocytes, macrophages and eosinophils following the loss of surface epithelial cells. Clin. Exp. Immunol. 109, 377-386.
11. Okada, S., Kita, H., George, T.J., Gleich, G.J., Leiferman, K.M., 1997. Migration of eosinophils through basement membrane components in vitro: Role of matrix metalloproteinase-9. Am. J. Respir. Cell Mol. Biol. 17, 519-528.
12. Scott, R.A.H., Lauweryns, B., Snead, D.M.J., Haynes, R.J., Mahida, Y., Dua, H.S., 1997. E-cadherin distribution and epithelial basement membrane characteristics of the normal human conjunctiva and cornea. Eye 11, 607-612.
13. Wallis, S., Lloyd, S., Wise, I., Ireland, G., Fleming, T.P., Garrod, D., 2000. The α isoform of protein kinase C is involved in signalling the response of desmosomes to wounding in cultured epithelial cells. Mol. Biol. Cell 11, 1077-1092.