The glycosylphosphatidylinositol (GPI) signal sequence of human placental alkaline phosphatase is not recognized by human Gpi8p in the context of the yeast GPI anchoring machinery

Molecular Microbiology

Volumn 46, Issue 3, 2002, Pages 745-748

  Meyer, Urs ^a,b   Fraering, Patrick ^a   Bosson, Régine ^a   Imhof, Isabella ^a   Benghezal, Mohammed ^a,c   Vionnet, Christine ^a   Conzelmann, Andreas ^a  

^a UNIVERSITY OF FRIBOURG   (Switzerland)

^b ZLB Bioplasma AG   (Switzerland)

^c UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE PLACENTA ISOENZYME; AMIDINOTRANSFERASE; GLYCOSYLPHOSPHATIDYLINOSITOL; PROTEIN; SIGNAL PEPTIDE;

ARTICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; TAXONOMIC IDENTIFICATION; YEAST; YEAST CELL;

ALKALINE PHOSPHATASE; AMINOACYLTRANSFERASES; CELL ADHESION MOLECULES; FEMALE; GLYCOSYLPHOSPHATIDYLINOSITOLS; HUMANS; PLACENTA; PREGNANCY; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; YEASTS;

EUKARYOTA;

EID: 0036441238     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.03192.x     Document Type: Article

References (6)

1. Benghezal, M., Benachour, A., Rusconi, S., Aebi, M., and Conzelmann, A. (1996) Yeast Gpi8p is essential for GPI anchor attachment onto proteins. EMBO J 15: 6575-6583.
2. De Sampaïo, G., Bourdineaud, J.P., and Lauquin, G.J. (1999) A constitutive role for GPI anchors in Saccharomyces cerevisiae: Cell wall targeting. Mol Microbiol 34: 247-256.
3. Meyer, U., Benghezal, M., Imhof, I., and Conzelmann, A. (2000) Active site determination of Gpi8p, a caspase-related enzyme required for glycosylphosphatidylinositol anchor addition to proteins. Biochemistry 39: 3461-3471.
4. Ohishi, K., Inoue, N., Maeda, Y., Takeda, J., Riezman, H., and Kinoshita, T. (2000) Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins. Mol Biol Cell 11: 1523-1533.
5. Schreuder, M.P., Brekelmans, S., van den Ende, H., and Klis, F.M. (1993) Targeting of a heterologous protein to the cell wall ofSaccharomyces cerevisiae. Yeast 9: 399-409.
6. Van der Vaart, J.M., te Biesebeke, R., Chapman, J.W., Toschka, H.Y., Klis, F.M., and Verrips, C.T. (1997) Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins. Appl Environ Microbiol 63: 615-620.