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Volumn 62, Issue 6, 2002, Pages 405-412

LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR)

Author keywords

IL 1 ; IL 12 ; IL 6; Quantitative competitive RT PCR

Indexed keywords

COMPLEMENTARY DNA; INTERLEUKIN 12; INTERLEUKIN 1BETA; INTERLEUKIN 6; LIPOPOLYSACCHARIDE; MESSENGER RNA;

EID: 0036430333     PISSN: 00365513     EISSN: None     Source Type: Journal    
DOI: 10.1080/00365510260389958     Document Type: Article
Times cited : (7)

References (8)
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    • Serth J, Panitz F, Herrmann H, Alves J. Single-tube nested competitive PCR with homologous competitor for quantitation of DNA target sequences: Theoretical description of heteroduplex formation, evaluation of sensitivity, precision and linear range of the method. Nucleic Acids Res 1998; 26: 4401-8.
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  • 2
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    • Simultaneous quantitation of multiple cytokine mRNAs by RT-PCR utilizing plate based EIA methodology
    • Hockett RD, Janowski KM, Bucy RP. Simultaneous quantitation of multiple cytokine mRNAs by RT-PCR utilizing plate based EIA methodology. J Immunol Methods 1995; 187: 273-85.
    • (1995) J Immunol Methods , vol.187 , pp. 273-285
    • Hockett, R.D.1    Janowski, K.M.2    Bucy, R.P.3
  • 3
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000; 25: 169-93.
    • (2000) J Mol Endocrinol , vol.25 , pp. 169-193
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  • 4
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    • In vitro cytokine mRNA expression in normal human peripheral blood mononuclear cells
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    • Barth, S.1    Kleinhappl, B.2    Gutschi, A.3    Jelovcan, S.4    Marth, E.5
  • 5
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    • A commentary on the practical applications of competitive PCR
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  • 7
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    • Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR
    • McDowell DG, Burns NA, Parkes HC. Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR. Nucleic Acids Res 1998; 26: 3340-7.
    • (1998) Nucleic Acids Res , vol.26 , pp. 3340-3347
    • McDowell, D.G.1    Burns, N.A.2    Parkes, H.C.3
  • 8
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    • A method for quantification of absolute amounts of nucleic acids by (RT)-PCR and a new mathematical model for data analysis
    • Vu HL, Troubetzkoy S, Nguyen HH, Russell MW, Mestecky J. A method for quantification of absolute amounts of nucleic acids by (RT)-PCR and a new mathematical model for data analysis. Nucleic Acids Res 2000; 28: E18.
    • (2000) Nucleic Acids Res , vol.28
    • Vu, H.L.1    Troubetzkoy, S.2    Nguyen, H.H.3    Russell, M.W.4    Mestecky, J.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.