Purification of the basic protein avidin using Gradiflow technology

Protein Expression and Purification

Volumn 26, Issue 1, 2002, Pages 149-152

  Rothemund, Deborah L ^a   Thomas, Theresa M ^a   Rylatt, Dennis B ^a  

^a Gradipore Ltd   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

GALLUS GALLUS;

AVIDIN; EGG WHITE; LYSOZYME;

ANIMAL; ARTICLE; CHICKEN; ELECTROPHORESIS; INSTRUMENTATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PH;

ANIMALS; AVIDIN; CHICKENS; EGG WHITE; ELECTROPHORESIS; HYDROGEN-ION CONCENTRATION; MURAMIDASE;

EID: 0036425271     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00517-X     Document Type: Article

References (8)

1. M.D. Melamed, N.M. Green, Avidin. 2. Purification and composition, Biochem. J. 89 (1963) 591-599.
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3. J. Margolis, G. Corthals, Z.C. Horvath, Preparative reflux electrophoresis, Electrophoresis 16 (1995) 98-100.
4. D.B. Rylatt, M. Napoli, D. Ogle, A. Gilbert, Electrophoretic transfer of proteins across polyacrylamide membranes, J. Chromatogr. 865 (1999) 133-145.
5. G. Henry, G.A. Orr, The purification of avidin and its derivatives on 2-Iminobiotin-6-aminohexyl-Sephrarose 4B, Anal. Biochem. 114 (1981) 92-96.
6. P. Cuatrecasas, M. Wilcheck, Single step purification of avidin from egg white by affinity chromatography on biocytin-Sepharose columns, Biochem. Biophys. Res. Commun. 33 (2) (1968) 235-239.
7. G.A. Orr, G.C. Henry, R. Zeheb, Purification of avidin and its derivatives on 2-iminobiotin-6-aminohexyl-Sephrarose 4B, Methods Enzymol. 22 (1986) 83-87.
8. V.E. Piskarev, A.M. Shuster, A.G. Gabibov, A.G. Rabinkov, A novel preparative method for the isolation of avidin and riboflavin-binding glycoprotein from chicken egg-white by the use of high-performance liquid chromatography, Biochem. J. 265 (1990) 301-304.