Crystallization and preliminary X-ray diffraction studies of HutP protein: An RNA-binding protein that regulates the transcription of hut operon in Bacillus subtilis

Journal of Structural Biology

Volumn 138, Issue 3, 2002, Pages 237-240

  Kumarevel, T S ^a   Fujimoto, Z ^b   Padmanabhan, B ^c   Oda, M ^a   Nishikawa, S ^a   Mizuno, H ^b   Kumar, P K R ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Anti termination; Crystallization; HutP protein; RNA binding protein; X ray crystallography

Indexed keywords

BACTERIAL PROTEIN; CIS ACTING ELEMENT; MESSENGER RNA; MUTANT PROTEIN; PROTEIN HUTP; RNA BINDING PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BINDING AFFINITY; CRYSTALLIZATION; DIFFUSION; GENE EXPRESSION REGULATION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; TRANSCRIPTION REGULATION; VAPOR; WILD TYPE; X RAY DIFFRACTION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; OPERON; RNA-BINDING PROTEINS; X-RAY DIFFRACTION;

BACILLUS SUBTILIS;

EID: 0036422627     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1047-8477(02)00024-2     Document Type: Article

References (17)

1. Arnaud, M.D., Debarbouille, M., Rapoport, G., Saier Jr., M.H., Reizer, J., 1996. In vitro reconstitution of transcriptional attenuation by the SacT and SacY proteins of Bacillus subtilis. J. Biol. Chem. 271, 18966-18972.
2. Aymerich, S., Steinmetz, M., 1992. Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BgiG/SacY family. Proc. Natl. Acad. Sci. USA 89, 10410-10414.
3. Babitzke, P., Yanofsky, C., 1993. Reconstitution of Bacillus subtilis trp attenuation in vitro with TRAP, the trp RNA-binding attenuation protein. Proc. Natl. Acad. Sci. USA 90, 133-137.
4. Berman, H.M., Bhat, T.N., Bourne, P.E., Feng, Z., Gilliland, G., Weissig, H., Westbrook, J., 2000. The protein data bank and the challenge of structural genomics. Nat. Struct. Biol. 1 (Suppl. 1), 957-959.
5. Chasin, L.A., Magasanik, B., 1968. Induction and repression of the histidine-degrading enzymes of Bacillus subtilis. J. Biol. Chem. 243, 5165-5178.
6. Houman, F., Diaz-Torres, M.R., Wright, A., 1990. Transcriptional antitermination in the bgl operon of E. coli is modulated by a specific RNA binding protein. Cell 62, 1153-1163.
7. Kimmhi, Y., Magasanik, B., 1970. Genetic basis of histidine degradation in Bacillus subtilis. J. Biol. Chem. 245, 3545-3548.
8. Lu, Y., Turner, R.J., Switzer, R.L., 1996. Function of RNA secondary structures in transcriptional attenuation of the Bacillus subtilis pyr operon. Proc. Natl. Acad. Sci. USA 93, 14462-14467.
9. Matthews, B.W., 1968. Solvent content of protein crystals. J. Mol. Biol. 33, 491-497.
10. McPherson, A., 1990. Current approaches to macromolecular crystallization. Eur. J. Biochem. 189, 1-23.
11. Oda, M., Sugishita, A., Furukawa, K., 1988. Cloning and nucleotide sequences of histidase and regulatory genes in the Bacillus subtilis hut operon and positive regulation of the operon. J. Bacteriol. 170, 3199-3205.
12. Oda, M., Katagai, T., Tomura, D., Shoun, H., Hoshino, T., Furukawa, K., 1992. Analysis of the transcriptional activity of the hut promoter in Bacillus subtilis and identification of a cis-acting regulatory region associated with catabolite repression downstream from the site of transcription. Mol. Microbiol. 6, 2573-2582.
13. Oda, M., Kobayashi, N., Ito, A., Kurusu, Y., Taira, K., 2000. Cis-acting regulatory sequences for antitermination in the transcript of the Bacillus subtilis hut operon and histidine-dependent binding of HutP to the transcript containing the regulatory sequences. Mol. Microbiol. 35, 1244-1254.
14. Otwinowski, Z., Minor, W., 1997. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307-326.
15. Sakabe, N., 1991. X-ray diffraction data collection system for modern protein crystallography with a Weissenberg camera and an imaging plate using synchrotron radiation. Nucl. Instrum. Methods Phys. Res. A 303, 448-463.
16. Wray Jr., L.V., Fisher, S.H., 1994. Analysis of Bacillus subtilis hut operon expression indicates that histidine-dependent induction is mediated primarily by transcriptional antitermination and that amino acid repression is mediated by two mechanisms: Regulation of transcription initiation and inhibition of histidine transport. J. Bacteriol. 176, 5466-5473.
17. Yoshida, K., Sano, H., Seki, S., Oda, M., Fujimura, M., Fujita, Y., 1995. Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome containing the hut and wapA loci. Microbiology 141, 337-343.