Extraction efficiencies and determination of ergosterol in a variety of environmental matrices

Communications in Soil Science and Plant Analysis

Volumn 33, Issue 15-18, 2002, Pages 3261-3275

  Verma, Brij ^a   Robarts, Richard D ^a   Headley, John V ^a   Peru, K M ^a   Christofi, N ^b  

^a ENVIRONMENT CANADA   (Canada)

^b EDINBURGH NAPIER UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ERGOSTEROL; TOLUENE; WATER;

ERGOSTEROL; EXTRACTION METHOD; FUNGUS; SOIL ANALYSIS;

AQUATIC ENVIRONMENT; CONFERENCE PAPER; DECOMPOSITION; DRUG ISOLATION; DRY MASS; FUNGAL BIOMASS; FUNGAL CELL; FUNGUS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PLANT FUNGUS INTERACTION; PLANT LEAF; PLANT STEM; SAPONIFICATION; WATER SAMPLING; WILLOW;

EID: 0036420483     PISSN: 00103624     EISSN: None     Source Type: Journal    
DOI: 10.1081/CSS-120014521     Document Type: Article

References (17)

1. Newell, S.Y.; Moran, M.A.; Wicks, R.; Hodson, R.E. Productivities of microbial decomposers during early stages of decomposition of leaves of a freshwater sedge. Freshw. Biol. 1995, 34, 135-148.
2. Suberkropp, K.; Weyers, H. Application of fungal and bacterial production methodologies to decomposing leaves in streams. Appl. Environ. Microbiol. 1996, 62, 1610-1615.
3. Kuehn, K.A.; Lemke, M.J.; Suberkropp, K.; Wetzel, R.G. Microbial biomass and production associated with decaying leaf litter of the emergent macrophyte Juncus effusus. Limnol. Oceanogr. 2000, 45 (4), 862-870.
4. Gessner, M.O.; Newell, S.Y. Bulk quantitative methods for the examination of eukaryotic organoosmotrophs in plant litter. Manual of Environmental Microbiology; ASM Press: Washington, DC, 1997; Chap. 31, 295-308.
5. Nielsen, K.F.; Madsen, J.O. Determination of ergosterol on mouldy building materials using isotope dilution and gas chromatography-tandem mass spectrometry. J. Chromatogr. A 2000, 898, 227-234.
6. Grant, W.D.; West, A.W. Measurement of ergosterol, diaminopimelic acid and glucosamine in soil: Evaluation as indicators of microbial biomass. J. Microbiol. Methods 1986, 6, 47-53.
7. Stahl, P.D.; Parkin, T.B. Relationship of soil ergosterol concentration and fungal biomass. Soil Biol. Biochem. 1996, 28, 847-855.
8. Suberkropp, K. Annual production of leaf-decaying fungi in a woodland stream. Freshw. Biol. 1997, 38, 169-178.
9. Arnezeder, C.; Koliander, W.; Hampel, W.A. Rapid determination of ergosterol in yeast cells. Anal. Chim. Acta 1985, 225, 129-136.
10. Rodriguez, R.J.; Parks, L.W. High performance liquid chromatography of sterols: Yeast sterols. Methods Enzymol. 1985, 111, 37-51.
11. Saraf, A.; Larson, L.; Burge, H.; Milton, D. Quantification of ergosterol and 3-hydroxy fatty acids in settled house dust by gas chromatography-mass spectrometry: Comparison with fungal culture and determination of endotoxin by a limulus amebocyte lysate assay. Appl. Environ. Microbiol. 1997, 63, 2554-2559.
12. Axelsson, B.O.; Saraf, A.; Larsson, L. Determination of ergosterol in organic dust by gas chromatography-mass spectrometry. J. Chromatogr. B Biomed. Appl. 1995, 666, 77-84.
13. Toh, T.H.; Prior, B.A.; van der Merwe, M.J. Quantification of plasma membrane ergosterol of saccharomyces cerevisiae by direct-injection atmospheric pressure chemical ionization/tandem mass spectrometry. Anal. Biochem. 2001, 288, 44-51.
14. Newell, S.Y. Membrane-containing fungal mass and fungal specific growth rate in natural samples. In Handbook of Methods in Aquatic Microbial Ecology; Kemp, B., Sherr, F., Sherr, B.E., Cole, J.J., Eds.; Lewis Publishers: Boca Raton, FL, 1993; 579-586.
15. Eash, N.S.; Stahl, P.D.; Parkin, T.B.; Karlen, D.L. A Simplified method for extraction of ergosterol from soil. Soil Sci. Soc. Am. J. 1996, 60, 468-471.
16. Rezanka, T.J. Analysis of sterol esters from alga and yeast by high-performance liquid chromatography and capillary gas chromatography - Mass spectrometry with chemical ionization. J. Chromatogr. 1992, 598, 219-236.
17. Newell, S.Y. Decomposition of shoots of a saltmarsh grass: Methodology and dynamics of microbial assemblages. Adv. Microb. Ecol. 1993, 13, 301-326.