Expression and purification of recombinant full-length NS3 protease - Helicase from a new variant of Hepatitis C virus

Protein Expression and Purification

Volumn 25, Issue 3, 2002, Pages 363-371

  Poliakov, Anton ^a   Hubatsch, Ina ^a   Shuman, Cynthia F ^a   Stenberg, Gun ^a   Danielson, U Helena ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; GLYCEROL; HEPACIVIRUS; INHIBITORY CONCENTRATION 50; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RNA HELICASES; RNA, VIRAL; SEQUENCE HOMOLOGY, NUCLEIC ACID; SODIUM CHLORIDE; VIRAL NONSTRUCTURAL PROTEINS;

ESCHERICHIA COLI; HEPATITIS C VIRUS;

EID: 0036412056     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00042-6     Document Type: Article

References (44)

1. World Health Organization, Hepatitis C - Global prevalence (update). Wkly. Epidemiol. Rec. 74 (49), (1999) 425-427.
2. K.J. Cummings, S.M. Lee, E.S. West, J. Cid-Ruzafa, S.G. Fein, Y. Aoki, et al., Interferon and ribavirin vs interferon alone in the re-treatment of chronic Hepatitis C previously nonresponsive to interferon: A meta-analysis of randomized trials, JAMA 285 (2) (2001) 193-199.
3. C.M. Rice, Flaviviridae: The viruses and their replication, in: B.N. Fields, D.M. Knipe, P.M. Howley (Eds.), Fields Virology, Lippincott-Raven Publishers, Philadelphia, PA, 1996, pp. 931-959.
4. Q.L. Choo, G. Kuo, A.J. Weiner, L.R. Overby, D.W. Bradley, M. Houghton, Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome, Science 244 (4902) (1989) 359-362.
5. R. Bartenschlager, L. Ahlborn-Laake, J. Mous, H. Jacobsen, Nonstructural protein 3 of the Hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions, J. Virol. 67 (7) (1993) 3835-3844.
6. M.R. Eckart, M. Selby, F. Masiarz, C. Lee, K. Berger, K. Crawford, et al., The Hepatitis C virus encodes a serine protease involved in processing of the putative nonstructural proteins from the viral polyprotein precursor, Biochem. Biophys. Res. Commun. 192 (2) (1993) 399-406.
7. L. Tomei, C. Failla, E. Santolini, R. De Francesco, N. La Monica, NS3 is a serine protease required for processing of Hepatitis C virus polyprotein, J. Virol. 67 (7) (1993) 4017-4026.
8. C. Failla, L. Tomei, R. De Francesco, Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins, J. Virol. 68 (6) (1994) 3753-3760.
9. J.L. Kim, K.A. Morgenstern, C. Lin, T. Fox, M.D. Dwyer, J.A. Landro, et al., Crystal structure of the Hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide, Cell 87 (2) (1996) 343-345.
10. R.A. Love, H.E. Parge, J.A. Wickersham, Z. Hostomsky, N. Habuka, E.W. Moomaw, et al., The crystal structure of Hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site, Cell 87 (2) (1996) 331-342.
11. G. Barbato, The solution structure of the N-terminal proteinase domain of the Hepatitis C virus (HCV) NS3 protein provides new insights into its activation and catalytic mechanism, J. Mol Biol. 289 (2) (1999) 371-384.
12. D.W. Kim, Y. Gwack, J.H. Man, J. Choe, C-terminal domain of the Hepatitis C virus NS3 protein contains an RNA helicase activity, Biochem. Biophys. Res. Commun. 215 (1) (1995) 160-166.
13. D.W. Kim, J. Kim, Y. Gwack, J.H. Han, J. Choe, Mutational analysis of the Hepatitis C virus RNA helicase, J. Virol. 71 (12) (1997) 9400-9409.
14. A.A. Kolykhalov, K. Mihalik, S.M. Feinstone, C.M. Rice, Hepatitis C virus-encoded enzymatic activities and conserved RNA elements in the 3′ nontranslated region are essential for virus replication in vivo, J. Virol 74 (4) (2000) 2046-20451.
15. B.W. Dymock, P.S. Jones, F.X. Wilson, Novel approaches to the treatment of Hepatitis C virus infection, Antivir. Chcm. Chcmolhcr. 11 (2) (2000) 79-96.
16. A.D. Kwong, J.L.. Kim, G. Rao, D. Lipovsek, S.A. Raybuck, Hepatitis C virus NS3/4A protease, Antiviral Res. 41 (1) (1999) 67-84.
17. S. Di Marco, M.. Rizzi, C. Volpari, M.A. Walsh, F. Narjes, S. Colarusso, et al., Inhibition of the hepatitis C virus NS3/4A protease. The crystal structures of two protease-inhibitor complexes, J. Biol. Chem. 275 (10) (2000) 7152.
18. J. Clough, A 'C' change for hepatitis treatment, Drug Discov. Today 7 (6) (2002) 335-336.
19. U. Koch, G. Biasiol, M. Brunetti, D. Fattori, M. Pallaoro, C. Steinkuhler, Role of charged residues in the catalytic mechanism of Hepatitis C virus NS3 protease: Electrostatic precollision guidance and transition-state stabilization, Biochemistry 40 (3) (2001) 631-640.
20. F. Narjes, M. Brunetti, S. Colarusso, B. Gerlach, U. Koch, G. Biasiol, et al., Alpha-ketoacids are potent slow binding inhibitors of the Hepatitis C virus NS3 protease, Biochemistry 39 (7) (2000) 1849-1861.
21. P. Ingallinella, E. Bianchi, R. Ingenito, U. Koch, C. Steinkuhler, S. Altamura, et al., Optimization of the P′-region of peptide inhibitors of Hepatitis C virus NS3/4A protease, Biochemistry 39 (42) (2000) 12898-12906.
22. M. Llinas-Brunet, M. Bailey, G. Fazal, E. Ghiro, V. Gorys, S. Goulet, et al., Highly potent and selective peptide-based inhibitors of the hepatitis C virus serine protease: Towards smaller inhibitors, Bioorg. Med. Chem. Lett. 10 (20) (2000) 2267-2270.
23. P. Gallinari, D. Brennan, C. Nardi, M. Brunetti, L. Tomei, C. Steinkuhler, et al., Multiple enzymatic activities associated with recombinant NS3 protein of Hepatitis C virus, J. Virol. 72 (8) (1998) 6758-6769.
24. S.S. Taremi, B. Beyer, M. Maher, N. Yao, W. Prosise, P.C. Weber, et al., Construction, expression, and characterization of a novel fully activated recombinant single-chain Hepatitis C virus protease, Protein Sci. 7 (1998) 2143-2149.
25. A.Y.M. Howe, R. Chase, S.S. Taremi, C. Risano, B. Beyer, B. Malcolm, et al., A novel recombinant single-chain Hepatitis C virus NS3-NS4A protein with improved helicase activity, Protein Sci. 8 (1999) 1322-1341.
26. N. Yao, P. Reichert, S.S. Taremi, W.W. Prosise, P.C. Weber, Molecular views of viral polyprotein processing revealed by the crystal structure of the Hepatitis C virus bifunctional protease-helicase, Structure 7 (11) (1999) 1353-1363.
27. P. Chomczynski, N. Sacchi, Single-step method of RNA isolation by acid guanidine thiocyanate-phenol-chloroform extraction, Anal. Biochem. 162 (1987) 156-159.
28. P. Chomczynski, A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples, Bioiechniques 15 (3) (1993) 532,536-534,537.
29. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning - A Laboratory Manual, Second Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989.
30. R.C. Kennedy, R.D. Henkel, D. Pauletti, J.S. Allan, T.H. Lee, M. Essex, et al., Antiserum to a synthetic peptide recognizes the HTLV-III envelope protein, Science 231 (1986) 1556-1559.
31. Y. Liu, W. Kati, C.-M. Chen, R. Tripathi, A. Molla, W. Kohlbrenner, Use of a fluorescent plate reader for measuring kinetic parameters with inner filter effect correction, Anal. Biochem. 267 (1999) 331-335.
32. E. Bianchi, A. Urbani, G. Biasiol, M. Brunetti, A. Pessi, R. De Francesco, et al., Complex formation between the Hepatitis C virus serine protease and a synthetic NS4A cofactor peptide, Biochemistry 36 (25) (1997) 7890-7897.
33. P. Ingallinella, S. Altamura, E. Bianchi, M. Taliani, R. Ingenito, R. Cortese, et al., Potent peptide inhibitors of human Hepatitis C virus NS3 protease are obtained by optimizing the cleavage products, Biochemistry 37 (25) (1998) 8906-8914.
34. G. Filocamo, L. Pacini, C. Nardi, L. Bartholomew, M. Scaturro, P. Delmastro, et al., Selection of functional variants of the NS3-NS4A protease of hepatitis C virus by using chimeric sindbis viruses, J. Virol. 73 (1) (1999) 561-575.
35. B.M. Beyer, R. Zhang, Z. Hong, V. Madison, B.A. Malcolm, Effect of naturally occurring active site mutations on Hepatitis C virus NS3 protease specificity, Proteins 43 (2) (2001) 82-88.
36. J. Cline, J.C. Braman, H.H. Hogrefe, PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases, Nucleic Acids Res. 24 (18) (1996) 3546-3551.
37. K.A. Morgenstern, J.A. Landro, K. Hsiao, C. Lin, Y. Gu, M.S. Su, et al., Polynucleotide modulation of the protease, nucleoside triphosphatase, and helicase activities of a Hepatitis C virus NS3-NS4A complex isolated from transfected COS cells, J. Virol. 71 (1997) 3767-3775.
38. D.L. Sali, R. Ingram, M. Wendel, D. Gupta, C. McNemar, A. Tsarbopoulos, et al., Serine protease of Hepatitis C virus expressed in insect cells as the NS3/4A complex, Biochemistry 37 (10) (1998) 3392-3401.
39. M.H. Alaoui-Ismaili, C. Gervais, S. Brunette, G. Gouin, M. Hamel, R.F. Rando, et al., A novel high troughput screening assay for HCV NS3 helicase activity, Antiviral Res. 46 (2000) 181-193.
40. C. Paolini, R. De Francesco, P. Gallinari, Enzymatic properties of Hepatitis C virus NS3-associated helicase, J. Gen. Virol. 81 (5) (2000) 1335-1345.
41. R. Banerjee, A. Dasgupta, Specific interaction of Hepatitis C virus protease/helicase NS3 with the 3′-terminal sequences of viral positive- and negative-strand RNA, J. Virol. 75 (4) (2001) 1708-1721.
42. M. Cerretani, L. Di Renzo, S. Serafini, A. Vitelli, N. Gennari, E. Bianchi, et al., A high-throughput radiometric assay for Hepatitis C virus NS3 protease, Anal. Biochem. 266 (2) (1999) 192-197.
43. M. Taliani, E. Bianchi, F. Narjes, M. Fossatelli, A. Urbani, C. Steinkuhler, et al., A continuous assay of Hepatitis C virus protease based on resonance energy transfer depsipeptide substrates, Anal. Biochem. 240 (1) (1996) 60-67.
44. A. Johansson, I. Hubatsch, E. Akerblom, G. Lindeberg, S. Winiwarter, U.H. Danielson, et al., Inhibition of Hepatitis C virus NS3 protease activity by product-based peptides is dependent on helicase domain, Bioorg. Med. Chem. Lett. 11 (2) (2001) 203-266.