Lifetime imaging with the zeiss LSM-510

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 4620, Issue , 2002, Pages 30-35

  Becker, Wolfgang ^a   Bergmann, Axel ^a   Weiss, Georg ^b  

^a BECKER AND HICKL GMBH   (Germany)

^b CARL ZEISS SMT AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOPHORES; FLUORESCENCE; LASER OPTICS; MULTIPHOTON PROCESSES; OPTICAL FIBERS; OPTICAL MICROSCOPY; PHOTODETECTORS; SCANNING;

LASER SCANNING MICROSCOPES (LSM);

IMAGING TECHNIQUES;

EID: 0036403842     PISSN: 0277786X     EISSN: None     Source Type: Journal    
DOI: 10.1117/12.470706     Document Type: Article

References (11)

1. M. Minsky: Memoir on inventing the confocal microscope. Scanning 10, 128-138 (1988).
2. J.G. White, W.B. Amos, M. Fordham: An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J Cell Biol 105, 41-48 (1987).
3. S. Tille: LSM 510 META. 6. Tag der Mikroskopie (2001).
4. J.R. Lakowicz, K.W. Berndt: Lifetime-selective fluorescence imaging using an rf phase-sensitive camera. Rev. Sci. Instrum. 62, 1727-1734 (1991).
5. A. Squire, P.J. Verveer, P.I.H. Bastiens: Multiple frequency fluorescence lifetime imaging microscopy. J. Microsc. 197, 136-149.
6. E.P. Buurman, R. Sanders, A. Draaijer, H.C. Gerritsen, J.J.F. van Veen, P.M. Houpt, Y.K. Levine: Fluorescence lifetime imaging using a confocal laser scanning microscope. Scanning 14, 155-159 (1992).
7. J. Sytsma, J.M. Vroom, C.J. de Grauw, H.C. Gerritsen, Time-Gated lifetime imaging and micro-volume spectroscopy using two-photon excitation. J. Microsc. 191, 39-51.
8. I. Bugiel, K. König, H. Wabnitz, Investigations of cells by fluorescence laser scanning microscopy with subnanosecond resolution. Lasers in the Life Sciences 3, 47-53 (1989).
9. W. Becker, A. Bergmann, K. Koenig, U. Tirlapur, Picosecond fluorescence lifetime microscopy by TCSPC imaging. Proc. SPIE 4262, 414-419.
10. Becker Hickl GmbH, SPC-300 through SPC-730 operating manual, www.becker-hickl.de.
11. W. Becker, A. Bergmann, H. Wabnitz, D. Grosenick, A. Liebert, High count rate multichannel TCSPC for optical tomography. Proc. SPIE 4431, 249-254.