Glycine 38 is crucial for the ribonucleolytic activity of human pancreatic ribonuclease on double-stranded RNA

Biochemical and Biophysical Research Communications

Volumn 297, Issue 2, 2002, Pages 390-395

  Gaur, Deepak ^a   Seth, Divya ^a   Batra, Janendra K ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Catalysis; Enzyme; Mutant; Recombinant protein; RNase

Indexed keywords

ALANINE; ASPARTIC ACID; DOUBLE STRANDED RNA; GLUTAMIC ACID; GLYCINE; PANCREATIC RIBONUCLEASE; RIBONUCLEASE A;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME PURIFICATION; MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CATTLE; CIRCULAR DICHROISM; GLYCINE; HUMANS; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; RIBONUCLEASE, PANCREATIC; RNA, DOUBLE-STRANDED;

BOVINAE;

EID: 0036402981     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)02216-7     Document Type: Article

References (29)

1. J.L. Weickmann, M. Elson, D.G. Glitz, Purification and characterization of human pancreatic ribonuclease, Biochemistry 20 (1981) 1272-1278.
2. J.J. Beintema, P. Wietzes, J. Weickmann, J.J. Glitz, The amino acid sequence of human pancreatic ribonuclease, Anal. Biochem. 136 (1984) 48-64.
3. M.R. Eftink, R.L. Biltonen, Pancreatic ribonuclease A: the most studied endoribonuclease, in: A. Neuberger, K. Brocklehurst (Eds.), Hydrolytic Enzymes, Elsevier, Amsterdam, 1987, pp. 333-376.
4. J.J. Beintema, H.J. Breukelman, A. Carsana, A. Furia, Evolution of vertebrate ribonucleases: ribonuclease A superfamily, in: G. D'Alessio, J.F. Riordan (Eds.), Ribonucleases Structures and Functions, Academic Press, New York, 1997, pp. 245-269.
5. A. Bardon, H. Sierakowska, D. Shugar, Human pancreatic-type ribonucleases with activity against double-stranded ribonucleic acids, Biochim. Biophys. Acta 438 (1976) 461-473.
6. M. Seno, J. Futami, M. Kosaka, S. Seno, H. Yamada, Nucleotide sequence encoding human pancreatic ribonuclease, Biochim. Biophys. Acta 1218 (1994) 466-468.
7. F.H. Westheimer, Pseudo-rotation in the hydolysis of phosphate esters, Acc. Chem. Res. 1 (1968) 70-78.
8. R.L. Campbell, G.A. Petsko, Ribonuclease structure and catalysis: crystal structure of sulfate-free native ribonuclease A at 1.5-A resolution, Biochemistry 26 (1987) 8554-8579.
9. M.W. Pandit, T. Ramakrishna, Helix-destabilization of deoxyribonucleic acid and poly[d(A-T) · d(A-T)] by bovine seminal ribonuclease, Biochim. Biophys. Acta 867 (1986) 1-8.
10. M. Libonati, A. Floridi, Breakdown of double-stranded RNA by bull seminal RNase, Eur. J. Biochem. 8 (1969) 81-87.
11. G. D'Alessio, A. Floridi, R. De Prisco, A. Pignero, E. Leone, Bull seminal ribonucleases I. Purification and physicochemical properties of the major component, Eur. J. Biochem. 26 (1972) 153-161.
12. T. Taniguchi, M. Libonati, Action of ribonuclease BS-I on a DNA-RNA hybrid, Biochem. Biophys. Res. Commun. 58 (1974) 280-286.
13. A. Bardon, H. Sierakowska, D. Shugar, Purification and properties of human acid-thermostable ribonucleases, and diagnosis of childhood pancreatic fibrosis, Clin. Chim. Acta 67 (1976) 231-243.
14. R. De Prisco, S. Sorrentino, E. Leone, M. Libonati, A ribonuclease from human seminal plasma active on double-stranded RNA, Biochim. Biophys. Acta 788 (1984) 356-363.
15. M. Libonati, S. Sorrentino, Revisiting the action of bovine ribonuclease A and pancreatic-type ribonucleases on double-stranded RNA, Mol. Cell. Biochem. 117 (1992) 139-151.
16. M. Libonati, J.J. Beintema, Basic charges on ribonuclease molecules and activity towards double-stranded polyribonucleotides, Biochem. Soc. Trans. 5 (1977) 470-474.
17. J.G. Opitz, M.I. Ciglic, M. Haugg, K. Trautwein-Fritz, S.A. Raillard, T.M. Jermann, S.A. Benner, Origin of the catalytic activity of bovine seminal ribonuclease against double-stranded RNA, Biochemistry 37 (1998) 4023-4033.
18. H.P. Bal, J.K. Batra, Human pancreatic ribonuclease: deletion of carboxyl-terminal EDST extension enhances ribonuclease activity and thermostability, Eur. J. Biochem. 245 (1997) 465-469.
19. T.A. Kunkel, J.D. Roberts, R.A. Zakour, Rapid and efficient site-specific mutagenesis without phenotypic selection, Meth. Enzymol. 154 (1987) 367-382.
20. F. Sanger, S. Nicklen, A.R. Coulson, DNA sequencing with chain-terminating inhibitors, Proc. Natl. Acad. Sci. USA 74 (1977) 5463-5467.
21. D. Gaur, S. Swaminathan, J.K. Batra, Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor: generation of inhibitor-resistant cytotoxic variants, J. Biol. Chem. 276 (2001) 24978-24984.
22. J. Buchner, I. Pastan, U. Brinkmann, A method for increasing the yield of properly folded recombinant fusion proteins: single-chain immunotoxins from renaturation of bacterial inclusion bodies, Anal. Biochem. 205 (1992) 263-270.
23. M.D. Bond, An in vitro binding assay for angiogenin using a placental ribonuclease inhibitor, Anal. Biochem. 173 (1988) 166-173.
24. E.M. Crook, A.P. Mathias, B.R. Rabin, Spectrophotometric assay of bovine Pancreatic Ribonuclease by the use of Cytidine 2′:3′-Phosphate, Biochem. J. 74 (1960) 234-238.
25. V. Cafaro, A. Bracale, A. Di Maro, S. Sorrentino, G. D'Alessio, A. Di Donato, New muteins of RNase A with enhanced antitumor action, FEBS Lett. 437 (1998) 149-152.
26. A. McPherson, G. Brayer, D. Cascio, R. Williams, The mechanism of binding of a polynucleotide chain to pancreatic ribonuclease, Science 232 (1986) 765-768.
27. M. Libonati, S. Sorrentino, Degradation of double-stranded RNA by mammalian pancreatic-type ribonucleases, Meth. Enzymol. 341 (2001) 234-248.
28. J. Pous, A. Canals, S.S. Terzyan, A. Guasch, A. Benito, M. Ribo, M. Vilanova, M. Coll, Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases, J. Mol. Biol. 303 (2000) 49-59.
29. S. Sorrentino, A. Carsana, A. Furia, J. Doskocil, M. Libonati, Ionic control of enzymic degradation of double-stranded RNA, Biochim. Biophys. Acta 609 (1980) 40-52.