Clinical evaluation of a NASBA-based assay for detection of Candida spp. in blood and blood cultures

Clinical Laboratory

Volumn 48, Issue 9-10, 2002, Pages 487-492

  Borst, Annemarie ^a   Verhoef, Jan ^a   Boel, Edwin ^a   Fluit, Ad C ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Blood culture; Candida spp.; Nucleic Acid Sequence Based Amplification (NASBA)

Indexed keywords

FUNGAL RNA;

ARTICLE; AUTOMATION; BLOOD ANALYSIS; BLOOD CULTURE; BLOOD SAMPLING; CANDIDA; CANDIDA ALBICANS; CANDIDA GLABRATA; CANDIDA GUILLIERMONDII; CANDIDA KRUSEI; CANDIDA LUSITANIAE; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; CANDIDA VISWANATHII; CANDIDIASIS; CLINICAL ARTICLE; CLINICAL EXAMINATION; CONTROLLED STUDY; FUNGUS IDENTIFICATION; HUMAN; INCUBATION TIME; NONHUMAN; NUCLEIC ACID AMPLIFICATION; OPPORTUNISTIC INFECTION; SENSITIVITY ANALYSIS; TIME;

CANDIDA; CANDIDIASIS; CHEMILUMINESCENT MEASUREMENTS; DNA PRIMERS; DNA PROBES; FUNGEMIA; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REAGENT KITS, DIAGNOSTIC; RNA, FUNGAL; SENSITIVITY AND SPECIFICITY;

CANDIDA; CANDIDA ALBICANS; CANDIDA GLABRATA; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; CANDIDA VISWANATHII; CLAVISPORA LUSITANIAE; FUNGI; ISSATCHENKIA ORIENTALIS; PICHIA GUILLIERMONDII;

EID: 0036402694     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Abi Said D, Anaissie E, Uzun O, Raad I, Pinzcowski H, Vartivarian S. The epidemiology of hematogenous candidiasis caused by different Candida species. Clin Infect Dis 1997; 24:1122-1128.
2. Wey SB, Mori M, Pfaller MA, Woolson RF, Wenzel RP. Hospital-acquired candidemia. The attributable mortality and excess length of stay. Arch Intern Med 1988; 148:2642-2645.
3. Giamarellou H, Antoniadou A. Epidemiology, diagnosis, and therapy of fungal infections in surgery. Infect Control Hosp Epidemiol 1996; 17:558-564.
4. Pittet D, Li N, Wenzel RP. Association of secondary and polymicrobial nosocomial bloodstream infections with higher mortality. Eur J Clin Microbiol Infect Dis 1993; 12:813-819.
5. Wenzel RP. Nosocomial candidemia: risk factors and attributable mortality. Clin Infect Dis 1995; 20:1531-1534.
6. Berenguer J, Buck M, Witebsky F, Stock F, Pizzo PA, Walsh TJ. Lysis-centrifugation blood cultures in the detection of tissue-proven invasive candidiasis. Disseminated versus single-organ infection. Diagn Microbiol Infect Dis 1993; 17:103-109.
7. Maksymiuk AW, Thongprasert S, Hopfer R, Luna M, Fainstein V, Bodey GP. Systemic candidiasis in cancer patients. Am J Med 1984; 77:20-27.
8. Borst A, Leverstein-Van Hall M, Verhoef J, Fluit A. Value of terminal subculture of automated blood cultures in patients with candidaemia. Eur J Clin Microbiol Infect Dis 2000; 19:803-805.
9. Shigei JT, Shimabukuro JA, Pezzlo MT, de la Maza LM, Peterson EM. Value of terminal subcultures for blood cultures monitored by BACTEC 9240. J Clin Microbiol 1995; 33:1385-1388.
10. Tinghitella TJ, Lamagdeleine MD. Assessment of Difco ESP 384 blood culture system by terminal subcultures: failure to detect Cryptococcus neoformans in clinical specimens. J Clin Microbiol 1995; 33:3031-3033.
11. Widjojoatmodjo MN, Borst A, Schukkink RA et al. Nucleic acid sequence-based amplification (NASBA) detection of medically important Candida species. J Microbiol Methods 1999; 38:81-90.
12. Borst A, Leverstein-Van Hall MA, Verhoef J, Fluit AC. Detection of Candida spp. in blood cultures using nucleic acid sequence-based amplification (NASBA). Diagn Microbiol Infect Dis 2001; 39:155-160.
13. Jarvis WR. Epidemiology of nosocomial fungal infections, with emphasis on Candida species. Clin Infect Dis 1995; 20:1526-1530.
14. Fluit AC, Jones ME, Schmitz FJ, Acar J, Gupta R, Verhoef J. Antimicrobial susceptibility and frequency of occurrence of clinical blood isolates in Europe from the SENTRY antimicrobial surveillance program, 1997 and 1998. Clin Infect Dis 2000; 30:454-460.
15. Tan CS, Wintermans RG, de Hoog GS, Engel HW, IJzerman EP. Shifts in the species spectrum of mycoses in the Netherlands from 1970-1990. Ned Tijdschr Geneeskd 1992; 136:631-637.
16. Rand KH, Houck H, Wolff M. Detection of candidemia by polymerase chain reaction. Mol Cell Probes 1994; 8:215-221.
17. Loeffler J, Hebart H, Bialek R et al. Contaminations occurring in fungal PCR assays. J Clin Microbiol 1999; 37:1200-1202.
18. Taylor GD, Buchanan-Chell M, Kirkland T, McKenzie M, Wiens R. Trends and sources of nosocomial fungaemia. Mycoses 1994; 37:187-190.
19. Loeffler J, Henke N, Hebart H et al. Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system. J Clin Microbiol 2000; 38:586-590.
20. Deiman B, Van Aerle P, Sillekens P. Characteristics and applications of nucleic acid sequence-based amplification (NASBA). Mol Biotechnol 2002; 20:163-179.