Generation of thermostable monomeric luciferases from Photorhabdus luminescens

Biochemical and Biophysical Research Communications

Volumn 296, Issue 5, 2002, Pages 1072-1076

  Westerlund Karlsson, Annette ^a   Saviranta, Petri ^b   Karp, Matti ^b  

^a ÅBO AKADEMI UNIVERSITY   (Finland)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

Bioluminescence; LuxAB; Peptide linker; Recombinant DNA; Translational fusion; Vibrio harveyi

Indexed keywords

BACTERIAL PROTEIN; LUCIFERASE; PROTEIN LUXA; PROTEIN LUXB; UNCLASSIFIED DRUG;

ARTICLE; CELL TYPE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PHOTORHABDUS LUMINESCENS; PRIORITY JOURNAL; PROTEIN EXPRESSION; TEMPERATURE; THERMOSTABILITY;

CHEMILUMINESCENT MEASUREMENTS; ENZYME STABILITY; ESCHERICHIA COLI; GENES, REPORTER; LUCIFERASES; PHOTORHABDUS; PLASMIDS; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PHOTORHABDUS; PHOTORHABDUS LUMINESCENS; VIBRIO; VIBRIO HARVEYI;

EID: 0036387079     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)02052-1     Document Type: Article

References (28)

1. J.W. Hastings, K.H. Nealson, Bacterial bioluminescence, Annu. Rev. Microbiol. 31 (1977) 549-595.
2. R. Belas, A. Mileham, D. Cohn, M. Hilmen, M. Simon, M. Silverman, Bacterial bioluminescence: isolation and expression of luciferase genes from Vibrio harveyi, Science 218 (1982) 791-793.
3. M. Boylan, J. Pelletier, E.A. Meighen, Fused bacterial luciferase subunits catalyze light emission in eucaryotes and procaryotes, J. Biol. Chem. 264 (1988) 1915-1918.
4. G. Kirchner, J.L. Roberts, G.D. Gustafson, T.D. Ingolia, Active bacterial luciferase from a fused gene: expression of a Vibrio harveyi luxAB translation in bacteria, yeast and plant cells, Gene 81 (1989) 349-354.
5. J. Lampinen, M. Korpela, P. Saviranta, R. Kroneld, M. Karp, Use of Escherichia coli cloned with genes encoding bacterial luciferase for evaluation of chemical toxicity, Toxic. Assessm. 5 (1990) 337-350.
6. J. Kurittu, S. Lönnberg, M. Virta, M. Karp, A group-specific microbiological test for the detection of tetracycline residues in raw milk, J. Agric. Food Chem. 48 (2000) 3372-3377.
7. W.H.R. Langridge, K.J. Fitzgerald, C. Koncz, J. Schell, A.A. Szalay, Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones, Proc. Natl. Acad. Sci. USA 86 (1989) 3219-3223.
8. O. Olsson, A. Escher, G. Sandberg, J. Schell, C. Koncz, A.A. Szalay, Engineering of monomeric bacterial luciferases by fusion of luxA and luxB genes in Vibrio harveyi, Gene 81 (1989) 335-347.
9. S. Koncz, W.H.R. Langridge, O. Olsson, J. Schell, A.A. Szalay, Bacterial and firefly luciferase genes in transgenic plants: advantages and disadvantages of a reporter gene, Dev. Genet. 11 (1990) 224-232.
10. A. Escher, D.J. O'Kane, A.A. Szalay, The β subunit polypeptide of Vibrio harveyi luciferase determines light emission at 42°C, Mol. Gen. Genet. 230 (1989) 385-393.
11. R.P. Hollis, C. Lagido, J. Pettitt, A.J. Porter, K. Killham, G.I. Paton, L.A. Glover, Toxicity of the bacterial luciferase substrate, n-decyl aldehyde, to Saccharomyces cerevisiae and Caenorhabditis elegans, FEBS Lett. 506 (2001) 140-142.
12. T.M. Schmidt, K. Kopecky, K.H. Nealson, Bioluminescence of the insect pathogen Xenorhabdus luminescens, Appl. Environ. Microbiol. 55 (1989) 2607-2612.
13. P. Colepicolo, K.-W. Cho, G.O. Poinar, J.W. Hastings, Growth and luminescence of bacterium Xenorhabdus luminescens from a human wound, Appl. Environ. Microbiol. 55 (1989) 2601-2606.
14. S. Frackman, M. Anhalt, K.H. Nealson, Cloning, organization, and expression of the bioluminescence genes of Xenorhabdus luminescens, J. Bacteriol. 172 (1990) 5767-5773.
15. T.C. Johnston, E.B. Rucker, L. Cochrum, K.S. Hruska, V. Vandegrift, The nucleotide sequence of the luxA and luxB genes of Xenerhabdus luminescens HM and a comparison of the amino acid sequences from four species of bioluminescent bacteria, Biochem. Biophys. Res. Commun. 170 (1990) 407-415.
16. J. Sambrook, E.F. Fritch, T. Maniatis, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1989.
17. C. Yanisch-Perron, J. Vieira, J. Messing, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors, Gene 33 (1985) 103-119.
18. A. Gunsalus-Miguel, E. Meighen, M. Ziegler-Nicoli, K. Nealson, J.W. Hastings, Purification and properties of bacterial luciferase, J. Biol. Chem. 247 (1972) 398-404.
19. M.J. Casadaban, S.N. Cohen, Analysis of gene control signals by DNA fusion and cloning in Escherichia coli, J. Mol. Biol. 138 (1980) 179-207.
20. R. Szittner, E. Meighen, Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestial bacterium, J. Biol. Chem. 265 (1990) 16581-16587.
21. A. Escher, D.J. O'Kane, J. Lee, A.A. Szalay, Bacterial luciferase αβ fusion protein is fully active as a monomer and highly sensitive in vivo to elevated temperature, Proc. Natl. Acad. Sci. USA 86 (1989) 6528-6532.
22. R.A. Hart, U. Rinas, J.E. Bailey, Protein composition of Vitreoscilla hemoglobin inclusion bodies produced in Escherichia coli, J. Biol. Chem. 265 (1990) 12728-12733.
23. J. Lampinen, M. Virta, M. Karp, Use of controlled luciferase expression for monitoring of chemicals affecting protein synthesis, Appl. Environ. Microbiol. 61 (1995) 2981-2989.
24. P. Saviranta, C. Oker-Blom, M. Karp, Baculovirus-mediated expression of bacterial and beetle luciferases in insect cells, in: A.A. Szalay, L.J. Kricka, P. Stanley (Eds.), Bioluminescence and Chemiluminescence, Status Report, Proceedings of the VIIth International Symposium on Bioluminescence and Chemiluminescence, Banff, Wiley, Chichester, NY, March 1993, pp. 94-98.
25. S. Zenno, K. Saigo, Identification of the genes encoding NAD(P)H-flavin oxidoreductases that are similar in sequence to Escherichia coli Fre in four species of luminous bacteria: Photorhabdus luminescens, Vibrio fischeri, Vibrio harveyi, and Vibrio orientalis, J. Bacteriol. 176 (1994) 3544-3551.
26. N. Kajiyama, E. Nakano, Thermostabilization of firefly luciferase by a single amino acid substitution at position 217, Biochemistry 32 (1993) 13795-13799.
27. N. Kajiyama, E. Nakano, Enhancement of thermostability of firefly luciferase from Luciola lateralis by a single amino acid substitution, Biosci. Biotechnol. Biochem. 58 (1994) 1170-1171.
28. P.J. White, D.J. Squirrell, P. Arnaud, C.R. Lowe, J.A.H. Murray, Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354, Biochem. J. 319 (1996) 343-360.