A novel cell-based scintillation proximity assay for studying protein function and activity in vitro using membrane-soluble scintillants

Biochemical and Biophysical Research Communications

Volumn 296, Issue 4, 2002, Pages 857-863

  Culliford, Steven J ^a,b   McCauley, Patrick ^a,c   Sutherland, Andrew J ^d   McCairn, Mark ^d   Sutherland, John ^e   Blackburn, Jonathan ^a,c   Kozlowski, Roland Z ^a,b  

^a Proteom Ltd   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d ASTON UNIVERSITY   (United Kingdom)

^e UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Cell membrane; Intact cells; Radioisotope assays; Scintillation proximity; SPA

Indexed keywords

CARBON 14; LIPOSOME; METHIONINE; OXADIAZOLE DERIVATIVE; RADIOLIGAND;

AQUEOUS SOLUTION; ARTICLE; BINDING ASSAY; CELL ASSAY; CELL CULTURE; CELL MEMBRANE; CELL MEMBRANE TRANSPORT; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; ISOTOPE LABELING; LIGAND BINDING; LIPID BILAYER; LIPOPHILICITY; MICROSOME; PRIORITY JOURNAL; PROTEIN FUNCTION; RADIOACTIVITY; RADIOASSAY; SCINTILLATION; SCREENING TEST; SOLUBILITY;

BIOCHEMISTRY; CELL MEMBRANE; HELA CELLS; HUMANS; LIPOSOMES; METHIONINE; MODELS, CHEMICAL; PHOSPHATIDYLCHOLINES; PROTEIN BINDING; PROTEINS; SCINTILLATION COUNTING; SOLVENTS; TIME FACTORS;

EID: 0036386377     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)00944-0     Document Type: Article

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