Scanning dual-color cross-correlation analysis for dynamic co-localization studies of immobile molecules

Single Molecules

Volumn 3, Issue 4, 2002, Pages 201-210

  Amediek, Axel ^a   Haustein, Elke ^a   Scherfeld, Dag ^a   Schwille, Petra ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

FCS; Fluorescence correlation spectroscopy; FRET; Imaging; Membranes; Single molecule

Indexed keywords

LIGAND; POLYACRYLAMIDE GEL; RECEPTOR;

ARTICLE; ARTIFACT; BLEACHING; CELL INTERACTION; CELL MEMBRANE; CHEMICAL INTERACTION; COMPLEX FORMATION; CONTROLLED STUDY; CORRELATION ANALYSIS; FLUORESCENCE SPECTROSCOPY; IMAGING SYSTEM; MEASUREMENT; MOLECULAR DYNAMICS; MOLECULAR PROBE; PHOTODYNAMICS; PIEZOELECTRICITY; SPECIES; VOLUMETRY;

EID: 0036385444     PISSN: 14385163     EISSN: None     Source Type: Journal    
DOI: 10.1002/1438-5171(200208)3:4<201::AID-SIMO201>3.0.CO;2-7     Document Type: Article

References (36)

1. Elson, E.L. Fluorescence correlation spectroscopy measures molecular transport in cells [Review]. Traffic 2 (2001), 789-796
2. Köhler, R.H., Schwille, P., Webb, W.W. & Hanson, M.R. Active protein transport through plastid tubules: velocity quantified by fluorescence correlation spectroscopy. Journal of Cell Science 113 (2000), 3921-3930
3. Wiseman, P.W., Squier, J.A., Ellisman, M.H. & Wilson, K.R. Two-photon image correlation spectroscopy and image cross-correlation spectroscopy. Journal of Microscopy (Oxford) 200 (2000), 14-25
4. Goulian, M. & Simon, S.M. Tracking Single Proteins within Cells. Biophys. J. 79 (2000), 2188-2198
5. Harms, G.S., Cognet, L., Lommerse, P.H.M., Blab, G.A. & Schmidt, T. Autofluorescent Proteins in Single-Molecule Research: Applications to Live Cell Imaging Microscopy. Biophys. J. 80 (2001), 2396-2408
6. Seisenberger, G. et al. Real-Time Single-Molecule Imaging of the Infection Pathway of an Adeno-Associated Virus. Sci 294 (2001), 1929-1932
7. Kues, T., Peters, R. & Kubitscheck, U. Visualization and Tracking of Single Protein Molecules in the Cell Nucleus. Biophys. J. 80 (2001), 2954-2967
8. Bacia, K., Majoul, I.V. & Schwille, P. Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis. Biophys. J. in press
9. Ambrose, W.P. et al. Single Molecule Fluorescence Spectroscopy at Ambient Temperature. Chem. Rev. 99 (1999), 2929-2956
10. Weiss, S. Fluorescence Spectroscopy of Single Biomolecules. Science 283 (1999), 1676-1683
11. Rigler, R. & Widengren, J. Ultrasensitive detection of single molecules by fluorescence correlation spectroscopy. BioScience, ed. B. Klinge & C. Owman (1990), pp. 180ff. Lund University Press, Lund.
12. Schwille, P., Bieschke, J. & Oehlenschläger, F. Kinetic Investigations by Fluorescence Correlation Spectroscopy: The Analytical and Diagnostic Potential of Diffusion Studies. Biophys. Chem. 66 (1997), 211-228
13. Dittrich, P.; Malvezzi-Campeggi, F.; Jahnz, M. & Schwille, P. Accessing molecular dynamics in cells by fluorescence correlation spectroscopy. Biol. Chem. 382 (2001), 491-494
14. Schwille, P., Meyer-Almes, F.J. & Rigler, R. Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J. 72 (1997), 1878-1886
15. Schwille, P. & Heinze, K.G. Two-photon fluorescence cross-correlation spectroscopy. Chemphyschem 2 (2001), 269-272
16. Rarbach, M., Kettling, U., Koltermann, A. & Eigen, M. Dual-color fluorescence cross-correlation spectroscopy for monitoring the kinetics of enzyme-catalyzed reactions. Methods 24 (2001), 104-116
17. Schwille, P. Fluorescence Correlation Spectroscopy and Its Potential for Intracellular Applications. Cell Biochem.Biophys. 34 (2001), 383-408
18. Widengren, J. & Rigler, R. Mechanisms of photobleaching investigated by fluorescence correlation spectroscopy. Bioimaging 4 (1996), 149-157
19. Eggeling, C., Widengren, J., Rigler, R. & Seidel, C. A.M. Photobleaching of Fluorescent Dyes under Conditions used for Single-Molecule-Detection: Evidence of Two-Step Photolysis. Anal. Chem. 70 (1998), 2651-2659
20. Meyer, T. & Schindler, H. Particle Counting by Fluorescence Correlation Spectroscopy. Biophys J. 54 (1988), 983-993
21. Petersen, N.O. Scanning fluorescence correlation spectroscopy. I. Theory and simulation of aggregation measurements. Biophys. J. 49 (1986), 809-815
22. Kohl, T., Heinze, K.G, Kuhlemann, R., Koltermann, A. & Schwille, P. A Protease Assay For Two-Photon Cross-Correlation and FRET Analysis Based Solely on Fluorescent Proteins. Submitted
23. Dickson, R.M. et al. Single Molecules Solvated in Pores of Polyacrilamide Gels. Molecular Crystals & Liquid Crystals Science & Technology Section A-Molecular Crystals & Liquid Crystals 291 (1996), 31-39
24. Dickson, R.M., Norris, D.J., Tzeng, Y.L. & Moerner, W.E. Three-dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels. Science 274 (1996), 966-969
25. Schwille, P., Korlach, J. & Webb, W.W. Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry 36 (1999), 176-182
26. Schwille, P., Haupts, U., Maiti, S. & Webb, W.W. Molecular Dynamics in Living Cells Observed by Fluorescence Correlation Spectroscopy with One- and Two-Photon Excitation. Biophys. J. 77 (1999), 2251-2265
27. Elson, E.L. & Magde, D. Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers 13 (1974), 1-27
28. Magde, D., Elson, E.L. & Webb, W.W. Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers 13 (1974), 29-61
29. Aragón, S.R. & Pecora, R. Fluorescence Correlation Spectroscopy and Brownian Rotatonal Diffusion. Biopolymers 14 (1975), 119-138
30. Aragón, S.R. & Pecora, R. Fluorescence correlation spectroscopy as a probe of molecular dynamics. The Journal of Chemical Physics 64 (1976), 1791-1803
31. Thompson, N.L. Topics in Fluorescence Spectroscopy, Volume 1: Techniques. Lakowicz, J.R. (ed.), pp. 337-378 (Plenum Press, New York,1991)
32. Brinkmeier, M., Dörre, K., Stephan, J. & Eigen, M. Two beam cross correlation: A method to characterize transport phenomena in micrometer-sized structures. Anal. Chem. 71 (1999), 609-616
33. Edman, L. Theory of Fluorescence Correlation Spectroscopy on Single Molecules. J. Phys. Chem. A 104 (2000), 6165-6170
34. Magde, D., Webb, W.W. & Elson, E.L. Fluorescence Correlation Spectroscopy. III. Uniform Translation and Laminar Flow. Biopolymers 17 (1978), 361-376
35. Lounis, B., Deich, J., Rosell, F.I., Boxer, S.G.& Moerner, W.E. Photophysics of DsRed, a red fluorescent protein, from the ensemble to the single-molecule level. J. Phys. Chem B. 105 (2001), 5048-5054
36. Pramanik, A. & Rigler, R. Ligand-receptor interactions in the membrane of cultured cells monitored by fluorescence correlation spectroscopy. Biol. 382 (2001), 371-378