Using reverse transcription and a competitive polymerase chain reaction for quantification of α1B-adrenoceptor mRNA

Polish Journal of Pharmacology

Volumn 54, Issue 4, 2002, Pages 401-405

  Kreiner, Grzegorz ^a   Sanak, Marek ^a   Zelek Molik, Agnieszka ^a   Nalepa, Irena ^a  

^a INSTITUTE OF PHARMACOLOGY   (Poland)

Author keywords

1B adrenergic receptor mRNA; Northern blot; PCR; Rat brain; Reverse transcription

Indexed keywords

ALPHA 1A ADRENERGIC RECEPTOR; ALPHA 1B ADRENERGIC RECEPTOR; ALPHA 1D ADRENERGIC RECEPTOR; MESSENGER RNA; RECEPTOR SUBTYPE; ALPHA 1 ADRENERGIC RECEPTOR;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; HIPPOCAMPUS; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PREFRONTAL CORTEX; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; QUANTITATIVE ASSAY; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA HYBRIDIZATION; STEADY STATE; THALAMUS; ANIMAL; BRAIN; GENETICS; ISOLATION AND PURIFICATION; MALE; METABOLISM; METHODOLOGY; WISTAR RAT;

ANIMALS; BLOTTING, NORTHERN; BRAIN; MALE; RATS; RATS, WISTAR; RECEPTORS, ADRENERGIC, ALPHA-1; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0036383606     PISSN: 12306002     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Chomczynski, P.: A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques, 1993, 15, 532-537.
2. Current Protocols in Molecular Biology on CD - summer 2001. Wiley & Sons, Inc.
3. Duhl D.M., Gillespie D.D., Sulser F.: Ethidium bromide fluorescence of 28S ribosomal RNA can be used to normalize samples in Northern or dot blots when analyzing small drug-induced changes in specific mRNA. J. Neurosci. Meth., 1999, 42, 211-218.
4. Gilliland G., Perrin S., Bunn H.F.: Competitive PCR for quantification of mRNA. In: PCR Protocols. Eds. Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., Academic Press, San Diego, 1990, 60-69.
5. 1-adrenergic receptors revealed by chloroethylclonidine. Mol. Pharmacol., 1987, 32, 505-510.
6. 2+. Pharmacol. Rev., 1988, 40, 87-119.
7. 1A-adrenoceptor mRNA level in rat prefrontal cortex. Eur. J. Pharmacol., 2002, 444, 151-159.
8. 1-adrenoceptors in rat brain revealed by in situ hybridization experiments utilizing subtype-specific probes. J. Neurosci., 1994, 14, 4252-4268.
9. Scofield M.A., Liu F., Abel P.W., Jeffries W.B.: Quantification of steady state expression of mRNA for alpha-1 adrenergic receptor subtypes using reverse transcription and a competitive polymerase chain reaction. J. Pharmacol. Exp. Ther., 1995, 275, 1035-1042.
10. 1-Adrenoceptor subtypes. Eur. J. Pharmacol., 1999, 375, 261-276.