메뉴 건너뛰기




Volumn 94, Issue 2, 2002, Pages 166-171

Extremely simple, rapid, and highly efficient transformation method for the yeast Saccharomyces cerevisiae using glutathione and early log phase cells

Author keywords

Glutathione; Polyethylene glycol; Saccharomyces cerevisiae; Transformation; Yeast

Indexed keywords

CELLS; DNA; OXIDATION; PH EFFECTS; POLYETHYLENE GLYCOLS;

EID: 0036378318     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(02)80138-4     Document Type: Article
Times cited : (16)

References (14)
  • 1
    • 0020529962 scopus 로고
    • Transformation of intact yeast cells treated with alkali cations
    • Ito, H., Fukuda, Y., Murata, K., and Kimura, A.: Transformation of intact yeast cells treated with alkali cations. J. Bacteriol., 153, 163-168 (1983).
    • (1983) J. Bacteriol. , vol.153 , pp. 163-168
    • Ito, H.1    Fukuda, Y.2    Murata, K.3    Kimura, A.4
  • 3
    • 0025974216 scopus 로고
    • High-efficiency transformation of yeast by electroporation
    • Becker, O. M. and Guarente, L.: High-efficiency transformation of yeast by electroporation. Methods Enzymol., 194, 182-187 (1991).
    • (1991) Methods Enzymol. , vol.194 , pp. 182-187
    • Becker, O.M.1    Guarente, L.2
  • 4
    • 0022122705 scopus 로고
    • Primary structure of the Saccharomyces cerevisiae GAL7 gene
    • Tajima, M., Nogi, Y., and Fukusawa, T.: Primary structure of the Saccharomyces cerevisiae GAL7 gene. Yeast, 1, 267-277 (1985).
    • (1985) Yeast , vol.1 , pp. 267-277
    • Tajima, M.1    Nogi, Y.2    Fukusawa, T.3
  • 6
    • 0019473684 scopus 로고
    • High-frequency transformation of the fission yeast Schizosaccharomyces pombe
    • Beach, D. and Nurse, P.: High-frequency transformation of the fission yeast Schizosaccharomyces pombe. Nature (London), 290, 140-142 (1981).
    • (1981) Nature (London) , vol.290 , pp. 140-142
    • Beach, D.1    Nurse, P.2
  • 7
    • 0020631078 scopus 로고
    • Saccharomyces cerevisiae actin-Escherichia coli lacZ gene fusions: Synthetic-oligonucleotide-mediated deletion of the 309 base pair intervening sequence in the actin gene
    • Larson, G. P., Itakura, K., Ito, H., and Rossi, J. J.: Saccharomyces cerevisiae actin-Escherichia coli lacZ gene fusions: synthetic-oligonucleotide-mediated deletion of the 309 base pair intervening sequence in the actin gene. Gene, 22, 31-39 (1983).
    • (1983) Gene , vol.22 , pp. 31-39
    • Larson, G.P.1    Itakura, K.2    Ito, H.3    Rossi, J.J.4
  • 9
    • 0035379761 scopus 로고    scopus 로고
    • Natural transformation of Pseudomonas fluorescens and Agrobacterium tumefaciens in soil
    • Demaneche, S., Kay, E., Gourbiere, F., and Simonet, P.: Natural transformation of Pseudomonas fluorescens and Agrobacterium tumefaciens in soil. Appl. Environ. Microbiol., 67, 2617-2621 (2001).
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 2617-2621
    • Demaneche, S.1    Kay, E.2    Gourbiere, F.3    Simonet, P.4
  • 10
    • 0035408426 scopus 로고    scopus 로고
    • Natural transformation in mesophilic and thermophilic bacteria: Identification and characterization of novel, closely related competence genes in Acinetobacter sp. strain BD413 and Thermus thermophilus HB27
    • Friedrich, A., Hartsch, Y., and Averhoff, B.: Natural transformation in mesophilic and thermophilic bacteria: identification and characterization of novel, closely related competence genes in Acinetobacter sp. strain BD413 and Thermus thermophilus HB27. Appl. Environ. Microbiol., 67, 3140-3148 (2001).
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 3140-3148
    • Friedrich, A.1    Hartsch, Y.2    Averhoff, B.3
  • 12
    • 0032697140 scopus 로고    scopus 로고
    • DNA uptake in bacteria
    • Amith, H. O., Danner, D. B., and Deich, R. A.: Genetic transformation. Annu. Rev. Biochem., 50, 41-68 (1981). Dubnau, D.: DNA uptake in bacteria. Annu. Rev. Microbiol., 53, 217-244 (1999).
    • (1999) Annu. Rev. Microbiol. , vol.53 , pp. 217-244
    • Dubnau, D.1
  • 13
  • 14
    • 0035159370 scopus 로고    scopus 로고
    • Evaluation of biological and physical protection against nuclease degradation of caly-bound plasmid DNA
    • Demaneche, S., Jocteur-Monrozier, L., Quiquampoix, H., and Simonet, P.: Evaluation of biological and physical protection against nuclease degradation of caly-bound plasmid DNA. Appl. Environ. Microbiol., 67, 293-299 (2001).
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 293-299
    • Demaneche, S.1    Jocteur-Monrozier, L.2    Quiquampoix, H.3    Simonet, P.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.