Construction and preliminary screening of a human phage single-chain antibody library associated with gastric cancer

Journal of Surgical Research

Volumn 102, Issue 2, 2002, Pages 150-155

  He, Jin ^a   Zhou, Ge ^a   Liu, Kang Da ^a   Qin, Xin Yu ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

Genetic antibody; Phage display; Stomach neoplasms

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY LIBRARY; ANTIBODY SCREENING; ANTIBODY SPECIFICITY; ANTIBODY TITER; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CLINICAL ARTICLE; CONTROLLED STUDY; DNA LIBRARY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE; LYMPH NODE METASTASIS; MOLECULAR CLONING; PENICILLIN RESISTANCE; PHAGEMID; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPLEEN; STOMACH CANCER;

EID: 0036354861     PISSN: 00224804     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsre.2001.6298     Document Type: Article

References (14)

1. Identification of lymph node metastases in recurrent colorectal cancer
2. The genetic engineering of monoclonal antibodies
3. Production of human antibodies using bacteriophage
4. Antibodies stage a comeback in cancer treatment
5. The antiglobulin response to therapeutic antibodies
6. Man-made antibodies
7. Generating improved single-chain Fv molecules for tumor targeting
8. Oligonucleotide primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes
9. By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro
10. Evaluation of different lymphoid tissue sources for the construction of human immunoglobulin gene libraries
11. A single chain Fv derived from a filamentous phage library has distinct tumor targeting advantages over one derived from a hybridoma
12. Anti-melanoma antibodies from melanoma patients immunized with genetically modified autologous tumor cells: Selection of specific antibodies from single-chain Fv fusion phage libraries
13. Importance of the linker in expression of single-chain Fv antibody fragments: Optimisation of peptide sequence using phage display technology
14. Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule