Studies on singlet oxygen formation and UVA light-mediated photobleaching of the yellow chromophores in human lenses

Experimental Eye Research

Volumn 74, Issue 2, 2002, Pages 217-229

  Ortwerth, Beryl J ^a   Chemoganskiy, Vitaliy ^a   Olesen, P R ^a  

^a UNIVERSITY OF MISSOURI   (United States)

Author keywords

Aging pigments; Ascorbic acid; Human lens; Photobleaching; Singlet oxygen; Triplet state; Tryptophan; UVA light

Indexed keywords

ANION; ARGON; ASCORBIC ACID; CATION; FLUORINE DERIVATIVE; GLUTATHIONE; GUANIDINE; HISTIDINE; HYDROCHLORIC ACID; OXYGEN; RADICAL; SINGLET OXYGEN; TRYPTOPHAN;

AGING; AIR; ANAEROBIC METABOLISM; ARTICLE; BLEACHING; CATARACT; CHROMATOPHORE; COLOR; CONTROLLED STUDY; CORRELATION ANALYSIS; DISEASE COURSE; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; IN VITRO STUDY; LENS; LIGHT ABSORPTION; LIGHT INTENSITY; OXYGEN TISSUE LEVEL; PHOTODEGRADATION; PHOTOSENSITIVITY; PHOTOTOXICITY; PRIORITY JOURNAL; PROTEIN DEGRADATION; SOLUBILIZATION; SPECTRUM; TIME; TISSUE HOMOGENATE; ULTRASOUND; ULTRAVIOLET A RADIATION;

EID: 0036353739     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1006/exer.2001.1114     Document Type: Article

References (62)

1. Fluorescence studies on tryptophan and sulfhydryl group changes of bovine lens crystallins in a photodynamic system
2. The reaction of singlet oxygen with proteins, with special reference to crystallins
3. Spectrophotometric estimation of 3-OH L-kynurenine O-β-glucoside in the human lens
4. Non-tryptophan fluorescence of crystallins from normal and cataractous human lenses
5. Effect of pigmentation on GSH redox cycle antioxidant defense in whole as well as different regions of human cataractous lens
6. Major changes in ocular UV protection with age
7. Similarity of the yellow chromophores isolated from human cataracts with those from ascorbic acid-modified calf lens proteins: Evidence for ascorbic acid glycation during cataract formation
8. Riboflavin photodegradation and photosensitizing effects are highly dependent on oxygen and ascorbate concentrations
9. Light exposure and the risk of cortical, nuclear and posterior subcapsular cataracts: The pathologies ocularis Liees a l'Age (POLA) study
10. Changes to the proteins of the human lens nucleus in cataract
11. Time resolved spectroscopic studies on the intact human lens
12. Electron paramagnetic resonance and spin trapping investigations of the photoreactivity of human lens proteins
13. Photochemical and photophysical studies on human lens constituents
14. The optical properties of the anterior segment of the eye: Implications for cortical cataract
15. Influence of external calcium and glucose on internal total and ionized calcium in the rat lens
16. Is the lens canned?
17. Age-related changes in the absorption characteristics of the primate lens
18. Mechanisms of formation of the Maillard protein crosslink pentosidine. Glucose, fructose and ascorbate as pentosidine precursors
19. The diffusion of oxygen in the mammalian lens
20. + formation
21. Changes of lens crystallins photosensitized with tryptophan metabolites
22. Wavelength dependency of cataracts in albino mice following chronic exposure
23. Photosensitized formation of ascorbate radicals by chloroaluminum phthalocyanine tetrasulfonate: An electron spin resonance study
24. A new method for the detection of singlet oxygen in aqueous solution
25. In vivo measurements of oxygen
26. Spectroscopic evaluation and classification of the normal, aging and cataractous lens
27. Photodamage to calf lenses in vitro by excimer laser radiation at 308, 337 and 350 nm
28. Photoxidation of the nonenzymatic browning products in calf lens α-crystallin
29. The generation of superoxide anion by the UVA irradiation of human lens proteins
30. The generation of hydrogen peroxide by the UVA irradiation of human lens proteins
31. Quantitation of the singlet oxygen produced by UVA irradiation of human lens proteins
32. The aggregation of human lens proteins blocks the scavenging of UVA-generated singlet oxygen by ascorbic acid and GSH
33. Correlation of nuclear color and opalescence with protein S-thiolation in human lenses
34. 3-Hydroxykynurenine and 3-hydroxyanthranylic acid may act as endogenous antioxidants in the eye lens
35. Protein-associated pigments that accumulate in the brunescent eye lens. Identification of a quinone derivative
36. Sensitizer-induced conformational changes in lens crystallin-1. Photodynamic action of methylene blue and N-formylkynurenine on bovine α-crystallin
37. Recent developments in vision research: Light damage in cataract
38. Longterm development of lens opacities after exposure to ultraviolet irradiation at 300 nm
39. Reactivity of singlet oxygen toward amino acids and peptides
40. Singlet oxygen production correlates with His and Trp destruction in brunescent cataract water-insoluble proteins
41. UVA irradiation of human lens proteins produces residual oxidation of ascorbic acid even in the presence of high levels of GSH
42. Ascorbic acid-induced crosslinking of lens proteins: Evidence supporting a Maillard reaction
43. UVA photolysis using the protein-bound sensitizers present in human lens
44. The relative sensitizer activity of purified advanced glycation endproducts
45. β-carbolines that accumulate in human tissues may serve a protective role against oxidative stress
46. In vivo and photophysical studies on photoxidative damage to lens proteins and their protection by radioprotectors
47. Photoinduced electron transfer reactions of rose bengal and selected electron donors
48. Fluorescence in human lens
49. The nonoxidative degradation of ascorbic acid at physiological conditions
50. Glycation of lens protein by the oxidation products of ascorbic acid
51. Estimating the solar ultraviolet radiation exposure to an intraocular lens implant
52. Chemical determination of tryptophan
53. Decrease in vitamin C concentration in human lenses during cataract progression
54. Structure and mechanism of formation of human lens fluorophore LM-1. Relationship to vesperlysine A and the advanced Maillard reaction in aging, diabetes and cataractogenesis
55. Changes in human lens proteins during cataract formation
56. The state of sulfhydryl groups in normal and cataractous human lenses
57. Decolouration of the lens pigment in senile nuclear cataract
58. Fluorescent glucoside in the human lens
59. Sunlight exposure and risk of lens opacities in a population-based study
60. Distribution of two metabolically related fluorophores in human lens measured by laser microprobe
61. Photosensitized oxidation in the ocular lens: Evidence for photosensitizers endogenous to the human lens
62. Environmental near-UV radiation and cataracts