Gap junctions assemble in the presence of cytoskeletal inhibitors, but enhanced assembly requires microtubules

Experimental Cell Research

Volumn 275, Issue 1, 2002, Pages 67-80

  Johnson, Ross G ^a,b   Meyer, Rita A ^a,b   Li, Xin Ren ^a,b   Preus, Doris M ^a,b   Tan, Lana ^a,b   Grunenwald, Haiying ^a,b   Paulson, Alicia F ^a,b   Laird, Dale W ^a,b   Sheridan, Judson D ^a,b  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

Actin filament; Cell communication; Connexin43; Dye injection; Freeze fracture; Gap junction; Microtubule

Indexed keywords

CHOLESTEROL; COLCHICINE; CONNEXIN 43; CYTOCHALASIN B; FLUORESCENT DYE; FORSKOLIN; GAP JUNCTION PROTEIN; GREEN FLUORESCENT PROTEIN; LUMICOLCHICINE; NOCODAZOLE;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL AGGREGATION; CELL GROWTH; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; DRUG EFFECT; ELECTRON MICROSCOPY; FREEZE FRACTURE; GAP JUNCTION; GROWTH INHIBITION; HEPATOMA CELL; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; RAT; SIGNAL TRANSDUCTION;

EID: 0036343776     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.2002.5480     Document Type: Article

References (61)

1. The cellular Internet: On-line with connexins
2. Assembly of heteromeric connexons in guinea-pig liver en route to the Golgi apparatus, plasma membrane, and gap junctions
3. Connexin46 is retained as monomers in a trans-Golgi compartment of osteoblastic cells
4. Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER
5. Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells
6. Mutational analysis of gap junction formation
7. Gap junction formation: A self-assembly model involving membrane domains of lipid and protein
8. Gap junction formation between reaggregating Novikoff hepatoma cells
9. Junction formation and experimental modification
10. Channels in the junctions between cells
11. Reconstitution of native-type noncrystalline lens fiber gap junctions from isolated hemichannels
12. In vitro assembly of gap junctions
13. Intracellular trafficking pathways in the assembly of connexins into gap junctions
14. Effects of colchicine on gap junction formation during retinal neurogenesis
15. Clustering of Cx43 cell-to-cell channels into gap junction plaques: Regulation by cAMP and microfilaments
16. Loss of gap junction plaques and inhibition of intercellular communication in ilimaquinone-treated BICR-M1Rk and NRK cells
17. Studies on the formation of a permeable cell membrane junction: I. Coupling under various conditions of membrane contact - Effects of colchicine, cytochalsin B, Dinitrophenol
18. Gap junction assembly in the preimplantation mouse conceptus is independent of microtubules, microfilaments, cell flattening, cytokinesis
19. Prostaglandins change cell shape and increase intercellular gap junctions in osteoblast cultured from rat fetal calvaria
20. Alterations of tight gap junctions in mouse hepatocytes following administration of colchicine
21. In vitro rapid assembly of gap junctions is induced by cytoskeleton disruptors
22. Cyclic AMP and LDL trigger a rapid enhancement in gap junction assembly through a stimulation of connexin trafficking
23. Enhanced gap junction assembly following the elevation of cAMP requires full length Cx43
24. Gap junction protein connexin-43 interacts directly with microtubules
25. Actin filament bundles are associated with fiber gap junctions in the primate lens
26. Dynamics of astrocyte adhesion as analyzed by a combination of atomic force microscopy and immunocytochemistry
27. Evidence for the participation of actin microfilaments and bristle coats in the internalization of gap junction membrane
28. Gap junction and its cytoskeletal undercoats as involved in invagination-endocytosis
29. Direct association of the gap junction protein connexin-43 with ZO-1 in cardiac myocytes
30. The structure and regulation of tight junctions
31. Cadherins: A molecular family important in selective cell-cell adhesion
32. Regulation of connexin43-mediated gap junctional intercellular communication by Ca2+ in mouse epidermal cells is controlled by E-cadherin
33. Construction of epitheloid sheets by transfection of mouse sarcoma cells with cDNAs for chicken cell adhesion molecules
34. Expression of the gap junction protein connexin43 in embryonic chick lens: Molecular cloning, ultrastructural localization, and post-translational phosphorylation
35. Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies
36. Analysis of gap junctions and formation plaques between reaggregating Novikoff hepatoma cells
37. Enhanced gap junction formation with LDL and apolipoprotein B
38. Increased gap junction assembly between cultured cells upon cholesterol supplementation
39. Junctions between cancer cells in culture: Ultrastructure and permeability
40. Gap junctions between Novikoff hepatoma cells following dissociation and recovery in the absence of cell contact
41. Microtubule inhibitors differentially affect translational movement, cell surface expression, and endocytosis of transferrin receptors in K562 cells
42. Analyzing phorbol ester effects on gap junction communication: A dramatic inhibition of assembly
43. Rapid and reversible reduction of junctional permeability in cells infected with a temperature-sensitive mutant of avian sarcoma virus
44. Trafficking, assembly, and function of a connexin43-green fluorescent protein chimera in live mammalian cells
45. Clustering of connexin 43-enhanced green fluorescent protein gap junction channels and functional coupling in living cells
46. Quantitative analysis of low-resistance junctions between cultured cells and correlation with gap junctional areas
47. Effects of cytochalasin, phalloidin, and pH on the elongation of actin filaments
48. Disruption of microtubules reveals independent apical targeting mechanisms for G-protein-coupled receptors in polarized renal epithelial cells
49. Dynamics of the interphase mammalian Golgi complex as revealed through drugs producing reversible Golgi disassembly
50. Membrane effects of cytochalasin B
51. Extracellular calcium and cadherins regulate the process of gap junction assembly between cells in culture
52. The actin cytoskeleton is required for receptor-mediated endocytosis in mammalian cells
53. Junctional communication is induced in migrating capillary endothelial cells
54. Microtubule-dependent vesicle transport: Modulation of channel and transporter activity in liver and kidney
55. Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport
56. Multiple pathways in the trafficking and assembly of connexin 26, 32, and 43 into gap junction intercellular communication channels
57. Cyclic AMP modifies the cellular distribution of connexin43 and induces a persistent increase in the junctional permeability of mouse mammary tumor cells
58. The C-terminus of connexin43 is required for the up-regulation of gap junction assembly by cAMP
59. Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques
60. Pertussis toxin-sensitive G proteins regulate the distribution of connexin43 within cells
61. Properties and regulation of gap junctional hemichannels in the plasma membranes of cultured cells