Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 1. Encapsulation of marrow stromal osteoblasts in surface crosslinked gelatin microparticles

Biomaterials

Volumn 23, Issue 22, 2002, Pages 4359-4371

  Payne, Richard G ^a   Yaszemski, Michael J ^b   Yasko, Alan W ^c   Mikos, Antonios G ^a  

^a RICE UNIVERSITY   (United States)

^b MAYO CLINIC   (United States)

^c UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Bone tissue engineering; Cell transplantation; Gelatin microparticles; Injectable biomaterials; Marrow stromal osteoblasts; Poly(propylene fumarate); Temporary cell encapsulation

Indexed keywords

PHYSIOLOGIC FLUIDS;

BIOASSAY; CELLS; CROSSLINKING; DEGRADATION; DNA; ENCAPSULATION; GELATION; POLYMERS; TISSUE;

BIOMATERIALS;

3,3' DITHIOBIS(SUCCINIMIDYL PROPIONATE); ALKALINE PHOSPHATASE; GELATIN; OSTEOCALCIN; POLYMER;

ANIMAL CELL; ARTICLE; BONE MINERALIZATION; CELL COUNT; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELL ENCAPSULATION; CELL POPULATION; CELL PROLIFERATION; CELL PROTECTION; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CROSS LINKING; DEGRADATION; DISPERSION; DISSOLUTION; DNA DETERMINATION; DNA SYNTHESIS; ENZYME ASSAY; MALE; NONHUMAN; OSTEOBLAST; PARTICLE SIZE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DETERMINATION; RAT; TEMPERATURE; THICKNESS; TISSUE ENGINEERING;

NON-PROGRAMMATIC;

ALKALINE PHOSPHATASE; ANIMALS; BIOCOMPATIBLE MATERIALS; BONE SUBSTITUTES; CELL DIVISION; CROSS-LINKING REAGENTS; DNA; DRUG COMPOUNDING; DRUG DELIVERY SYSTEMS; GELATIN; INJECTIONS; OSTEOBLASTS; OSTEOCALCIN; PHENOTYPE; POLYMERS; RATS; RATS, SPRAGUE-DAWLEY; THYMIDINE; TIME FACTORS; TISSUE ENGINEERING;

EID: 0036342204     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0142-9612(02)00184-9     Document Type: Article

References (20)

1. The ingrowth of new bone tissue and initial mechanical properties of a degrading polymeric composite scaffold
2. In vitro degradation of a poly(propylene fumarate)/β-tricalcium phosphate composite orthopaedic scaffold
3. In vivo degradation of a poly(propylene fumarate)/β-tricalcium phosphate injectable composite scaffold
4. Crosslinking characteristics of an injectable poly(propylene fumarate)/β-tricalcium phosphate paste and mechanical properties of the crosslinked composite for use as a biodegradable bone cement
5. Design considerations in immunoisolation
6. Polyacrylate microcapsules for cell delivery
7. Xenotransplantation of cells using biodegradable microcapsules
8. Viability and protein secretion from human hepatoma (HepG2) cells encapsulated in 400-μm polyacrylate microcapsules by submerged nozzle-liquid jet extrusion
9. Immunoisolation
10. Non-antigenic and low allergic gelatin produced by specific digestion with an enzyme-coupled matrix
11. Bone regeneration by basic fibroblast growth factor complexed with biodegradable hydrogels
12. Osteoblast function on synthetic biodegradable polymers
13. Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds
14. Thermoreversible gelation of PEG-PLGA-PEG triblock copolymer aqueous solutions
15. Osteoblastic phenotype of rat marrow stromal cells cultured in the presence of dexamethasone, β-glycerolphosphate, and L-ascorbic acid
16. An optimized method for the chemiluminescent detection of alkaline phosphatase levels during osteodifferentiation by bone morphogenetic protein 2
17. Effects of biodegradable polymer particles on rat marrow-derived stromal osteoblasts in vitro
18. The developmental stages of osteoblast growth and differentiation exhibit selective responses of genes to growth factors (TGFβ1) and hormones (Vitamin D and Glucocorticoids)