A fast and sensitive nucleic acid extraction method for the detection of Cryptosporidium by PCR in environmental water samples

Water Science and Technology: Water Supply

Volumn 2, Issue 3, 2002, Pages 95-100

  Dellunde J ^a   Pina, S ^a   Jofre, J ^a   Lucena, F ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Cryptosporidium; Environmental; Excystation; PCR

Indexed keywords

EXTRACTION; PURIFICATION; SILICA; WATER;

EXCYSTATION;

NUCLEIC ACIDS;

GUANIDINE DERIVATIVE; NUCLEASE; NUCLEIC ACID; SILICON DIOXIDE; THIOCYANIC ACID DERIVATIVE;

EXTRACTION;

ANALYTIC METHOD; CONFERENCE PAPER; CRYPTOSPORIDIUM; DNA EXTRACTION; NONHUMAN; OOCYST; POLYMERASE CHAIN REACTION; PURIFICATION; WATER CONTAMINATION; WATER SAMPLING;

CRYPTOSPORIDIUM; PROTOZOA;

EID: 0036336291     PISSN: 16069749     EISSN: None     Source Type: Journal    
DOI: 10.2166/ws.2002.0090     Document Type: Conference Paper

References (11)

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4. Fast and reliable extraction of protozoan parasite DNA from fecal specimens
5. Bench scale experiments for the evaluation of a membrane filtration method for the recovery efficiency of Giardia and Cryptosporidium from water
6. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples
7. Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR
8. DNA sequence for the specific detection of Cryptosporidium parvum by the polymerase chain reaction
9. Comparison of primers and optimisation of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water
10. Parameters affecting polymerase chain reaction detection of waterborne Cryptosporidium parvum oocysts
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