Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B

Molecular and Cellular Biology

Volumn 22, Issue 17, 2002, Pages 6247-6260

  Scheid, Michael P ^a   Marignani, Paola A ^a   Woodgett, James R ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

2 MORPHOLINO 8 PHENYLCHROMONE; 4 HYDROXYTAMOXIFEN; ALANINE; ASPARTIC ACID; ENZYME; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; PROTEIN PDK1; SERINE; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INDUCTION; ENZYME LOCALIZATION; EXPRESSION VECTOR; GENE MUTATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; SITE DIRECTED MUTAGENESIS;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANIMALS; BINDING SITES; CATTLE; CELL LINE; CELL MEMBRANE; DIMERIZATION; ENZYME ACTIVATION; ENZYME INHIBITORS; HUMANS; INSULIN-LIKE GROWTH FACTOR I; KIDNEY; MEMBRANE PROTEINS; PHOSPHORYLATION; PHOSPHOSERINE; PHOSPHOTHREONINE; PROTEIN INTERACTION MAPPING; PROTEIN KINASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; RECEPTORS, ESTROGEN; RECOMBINANT FUSION PROTEINS; SIROLIMUS; TACROLIMUS BINDING PROTEIN 1A; TAMOXIFEN; TRANSFECTION;

VECTORS;

EID: 0036333737     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.17.6247-6260.2002     Document Type: Article

References (37)

1. Mechanism of activation of protein kinase B by insulin and IGF-1
2. Characterization of a 3-phosphoinositide-dependent protein kinase, which phosphorylates and activates protein kinase Bα
3. A versatile synthetic dimerizer for the regulation of protein-protein interactions
4. Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B
5. Role of translocation in the activation and function of protein kinase B
6. Domain swapping used to investigate the mechanism of protein kinase B regulation by 3-phosphoinositide-dependent protein kinase 1 and S473 kinase
7. Regulation of GLUT1 gene transcription by the serine/threonine kinase Akt1
8. Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase
9. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region
10. Identification of a pocket in the PDK1 kinase domain that interacts with PIF and the C-terminal residues of PKA
11. The PIF-binding pocket in PDK1 is essential for activation of S6K and SGK, but not PKB
12. Oncogenic kinase signalling
13. Two splice variants of protein kinase Bγ have different regulatory capacity depending on the presence or absence of the regulatory phosphorylation site serine 472 in the carboxyl-terminal hydrophobic domain
14. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction
15. Phosphorylation of Ser-241 is essential for the activity of 3-phosphoinositide-dependent protein kinase-1: Identification of five sites of phosphorylation in vivo
16. Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity
17. Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1
18. Phosphoinositide-3-OH kinase-dependent regulation of GSK-3 and PKB/AKT by the integrin linked kinase (ILK)
19. Effect of phosphoinositide-dependent kinase 1 on protein kinase B translocation and its subsequent activation
20. Direct regulation of the Akt proto-oncogene product by phosphatidylinositol-3,4-bisphosphate
21. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase
22. A phosphoserine-regulated docking site in the protein kinase RSK2 that recruits and activates PDK1
23. Insulin-stimulated protein kinase B phosphorylation on Ser-473 is independent of its activity and occurs through a staurosporine-insensitive kinase
24. Activation of protein kinase B/cAkt in hepatocytes is sufficient for the induction of expression of the gene encoding glucokinase
25. Construction and characterization of a conditionally active version of the serine/threonine kinase Akt
26. Var2 is required for cell spreading
27. Oncogenic transformation of cells by a conditionally active form of the protein kinase Akt/PKB
28. Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity
29. Involvement of the pleckstrin homology domain in the insulin-stimulated activation of protein kinase B
30. Phosphatidylinositol(3,4,5)P3 is essential but not sufficient for protein kinase B (PKB) activation: Phosphatidylinositol(3,4)P2 is required for PKB phosphorylation at Ser-473: Studies using cells from SH2-containing inositol-5-phosphatase knockout mice
31. PKB/AKT: Functional insights from genetic models
32. Evidence of insulin-stimulated phosphorylation and activation of the mammalian target of rapamycin mediated by a protein kinase B signaling pathway
33. Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B
34. Akt/protein kinase B is regulated by autophosphorylation at the hypothetical PDK-2 site
35. The P13K-PDK1 connection: More than just a road to PKB
36. Regulation of protein kinase B in rat adipocytes by insulin, vanadate, and peroxovanadate. Membrane translocation in response to peroxovanadate
37. The role of 3-phosphoinositide-dependent protein kinase 1 in activating AGC kinases defined in embryonic stem cells