Effective structure of a leader open reading frame for enhancing the expression of GC-rich genes

Journal of Biochemistry

Volumn 132, Issue 1, 2002, Pages 63-70

  Ishida, Masami ^a   Oshima, Tairo ^b  

^a TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

^b TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

Expression vector; GC rich gene; Overexpression; Thermostable protein; Thermus thermophilus

Indexed keywords

AMINO ACID; OLIGONUCLEOTIDE; PROTEIN SUBUNIT; TRYPTOPHAN SYNTHASE; BACTERIAL PROTEIN; PROTEIN POLA; PROTEIN TRPA; PROTEIN TRPB; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; ESCHERICHIA COLI; GC RICH SEQUENCE; GENE; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; THERMUS THERMOPHILUS; CONTROLLED STUDY; DNA SEQUENCE; EXPRESSION VECTOR; GENE MUTATION; GENE OVEREXPRESSION; GENETIC CODE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN MOTIF; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; START CODON;

AMINO ACIDS; BASE SEQUENCE; ESCHERICHIA COLI; GC RICH SEQUENCE; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, OVERLAPPING; MOLECULAR SEQUENCE DATA; MUTATION; OLIGONUCLEOTIDES; OPEN READING FRAMES; PLASMIDS; PROTEIN SUBUNITS; THERMUS THERMOPHILUS; TRYPTOPHAN SYNTHASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; THERMUS; THERMUS THERMOPHILUS; VECTORS;

EID: 0036327605     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003199     Document Type: Article

References (17)

1. Overexpression of genes of an extreme thermophile. Thermus thermophilus, in Escherichia coli cells
2. Highly efficient production of Thermus thermophilus enzymes: A practical method to overexpress in Escherichia coli of GC-rich genes from an extreme thermophile
3. Screening of a mutant plasmid with high expression efficiency of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus, in Escherichia coli
4. The tryptophan operon
5. Structure and expression of the ompB operon, the regulatory locus for the outer membrane porin regulon in Salmonella typhimurium
6. Translation initiation
7. Enhanced translational efficiency with two-cistron expression system
8. Cloning and sequence analysis of tryptophan synthase genes of an extreme thermophile, Thermus thermophilus HB27: Plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T thermophilus cells
9. Cloning, nucleotide sequence, and expression in Escherichia coli of DNA polymerase gene (polA) from Thermus thermophilus HB8
10. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase
11. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors
12. Tryptophan synthase from Escherichia coli and Salmonella typhimurium
13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
14. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
15. Codon usage tabulated from the international DNA sequence databases
16. Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli