Simultaneous isolation of keratinocytes and fibroblasts from a human cutaneous biopsy for the production of autologous reconstructed skin

Canadian Journal of Chemical Engineering

Volumn 79, Issue 4, 2001, Pages 663-667

  Rochon, Marie Hélène ^a   Gauthier, Marie Josée ^a   Auger, Francois A ^a,b   Germain, Lucie ^a,b  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

Culture; Fibroblasts; Keratinocytes; Reconstructed skin; Tissue engineering

Indexed keywords

ENZYME;

MEDICAL APPLICATION;

AUTOTRANSPLANTATION; CELL COUNT; CELL ISOLATION; CELL STRUCTURE; CELL ULTRASTRUCTURE; CELL VIABILITY; CONFERENCE PAPER; CONTROLLED STUDY; EPIDERMIS; EXTRACELLULAR MATRIX; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; KERATINOCYTE; SKIN BIOPSY; SKIN GRAFT; TISSUE ENGINEERING;

EID: 0036323946     PISSN: 00084034     EISSN: None     Source Type: Journal    
DOI: 10.1002/cjce.5450790427     Document Type: Article

References (8)

1. Improvement of human keratinocyte isolation and culture using thermolysin
2. Characterization of a tissue-engineered model for cutaneous wound healing
3. Elastin is expressed in a new model of in vitro reconstructed skin with remarkable mechanical properties in vitro and after grafting
4. Isolation and growth of adult human epidermal keratinocytes in cell culture
5. Epidermal and hair follicle transglutaminases. Partial characterization of soluble enzymes in newborn mouse skin
6. Characterization of a New tissue-engineered human skin equivalent with hair
7. A Method for the isolation and serial propagation of keratinocytes, endothelial cells, and fibroblasts from a single punch biopsy of human skin
8. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells