Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis

European Journal of Biochemistry

Volumn 269, Issue 12, 2002, Pages 2889-2896

  Mattern Dogru, Emine ^a   Ma, Xueyan ^a   Hartmann, Joachim ^a   Decker, Heinz ^a   Stöckigt, Joachim ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

hydrolase enzyme superfamily; Active site residues; Modelling; Polyneuridine aldehyde esterase; Site directed mutagenesis

Indexed keywords

AJMALINE; ESTERASE; HYDROXYNITRILE LYASE; INDOLE ALKALOID; MUTANT PROTEIN; POLYNEURIDINE ALDEHYDE ESTERASE; UNCLASSIFIED DRUG;

ALPHA CHAIN; ARTICLE; BETA CHAIN; DRUG SYNTHESIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME MECHANISM; ENZYME STRUCTURE; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN FAMILY; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

ACETONITRILES; ALDEHYDE-LYASES; AMINO ACID SEQUENCE; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; CRYSTALLOGRAPHY, X-RAY; ENZYME ACTIVATION; ENZYME INHIBITORS; HEVEA; INDOLE ALKALOIDS; KINETICS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RAUWOLFIA; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; HEVEA BRASILIENSIS;

EID: 0036315482     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02956.x     Document Type: Article

References (25)

1. Biosynthesis in Rauvolfia serpentina, modern aspects of an old medicinal plant
2. Klinische untersuchungen über die wirkungsweise des rauwolfia alkaloids (Ajmalin) bei herzrhythmusstörungen insbesondere der extrasystole
3. Alkaloid metabolism in plant cell culture
4. Polyneuridine aldehyde esterase: An unusually specific enzyme involved in the biosynthesis of sarpagine type alkaloids
5. Characterization of polyneuridine aldehyde esterase, a key enzyme in the biosynthesis of sarpagine/ajmaline type alkaloids
6. The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the α/β hydrolase super family
7. The defense-related rice gene Pir7b encodes an α/β hydrolase fold protein exhibiting esterase activity towards naphthol AS-esters
8. Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis
9. Purification, characterization, and cloning of α-hydroxynitrile lyase from cassava (Manihot esculenta Crantz)
10. The α/β hydrolase fold
11. Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis
12. Mechanism of cyanogenesis: The crystal structure of hydroxynitrile lyase from Hevea brasiliensis
13. DNA sequencing with chain-terminating inhibitors
14. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
15. Comparative protein modelling by satisfaction of spatial restraints
16. The CCP4 suite: Programs for protein crystallography
17. Technical note: An irreversible serine protease inhibitor
18. Modification of histidyl residues in proteins by diethylpyro-carbonate
19. Study of the (S)-hydroxynitrile lyase from Hevea brasiliensis: Mechanistic implications
20. Of barn owls and bankers: A lush variety of α/β hydrolases
21. Atomic structure of acetylcholinesterase from Torpedo californica: A prototypic acetylcholine-binding protein
22. Purification and partial characterisation of maize (Zea mays L.) β-glucosidase
23. Further additions to Molscript version 1.4. Including reading and contouring of electron-density maps
24. Raster3D - Photorealistic molecular graphics