Cloning of the pepX gene of Lactobacillus helveticus IFO3809 encoding salt-tolerant X-prolyl dipeptidyl aminopeptidase and characterization of the enzyme

Journal of Bioscience and Bioengineering

Volumn 93, Issue 6, 2002, Pages 589-594

  Kimura, Keitarou ^a   Nagasawa, Atsushi ^b   Fujii, Mikio ^b   Itoh, Yoshifumi ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b JAPAN TOBACCO INC   (Japan)

Author keywords

Lactobacillus; Peptidase; Proline; Salt tolerant

Indexed keywords

AMINO ACIDS; CHEMICAL BONDS; COLUMN CHROMATOGRAPHY; DNA SEQUENCES; ENCODING (SYMBOLS); ENZYMES; ESCHERICHIA COLI; FILTRATION; GENES; HYDROLYSIS; POSITIVE IONS;

FLANKING SEQUENCES;

CLONING;

AMINO ACID; AMINOPEPTIDASE; CATION; DIMER; GENE PRODUCT; OLIGOPEPTIDE; OLIGOPEPTIDE PERMEASE; PEPTIDE; PERMEASE; PROTEIN OPPA; PROTEIN PEPX; SODIUM CHLORIDE; TRANSPOSASE; UNCLASSIFIED DRUG; X PROLYL DIPEPTIDYL AMINOPEPTIDASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CHEMICAL BINDING; CONTROLLED STUDY; DIMERIZATION; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENETIC VARIABILITY; HYDROLYSIS; LACTOBACILLUS HELVETICUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROMOTER REGION; PROTEIN TRANSPORT; SALT TOLERANCE; SEQUENCE ANALYSIS;

ANIMALIA; ESCHERICHIA COLI; LACTOBACILLUS; LACTOBACILLUS HELVETICUS; LACTOBACILLUS SP.; POSIBACTERIA; PROKARYOTA;

EID: 0036308958     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.93.589     Document Type: Article

References (28)

1. The physiology and biochemistry of the proteolytic system in lactic acid bacteria
2. Genetics of the proteolytic system of lactic acid bacteria
3. Casein utilization by lactococci
4. Peptidases and amino acid catabolism in lactic acid bacteria
5. Genetic characterization of pepP, which encodes an aminopeptidase P whose deficiency does not affect Lactococcus lactis growth in milk, unlike deficiency of the X-prolyl dipeptidyl aminopeptidase
6. Synergistic action of an X-prolyl dipeptidyl aminopeptidase and a non-specific aminopeptidase in protein hydrolysis
7. Hydrolysis of αsl- and β-casein-derived peptides with a broad specificity aminopeptidase and proline specific aminopeptidases from Lactococcus lactis subsp, cremoris AM2
8. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
9. High efficiency transformation of Escherichia coli with plasmids
10. Peptidase mutants of Salmonella typhimurium
11. Purification and properties of glycylprolyl β-naphthylamidase in human submaxillary gland
12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
13. Cloning of peptidase genes from Lactobacillus helveticus CNRZ 32
14. X-prolyl dipeptidyl aminopeptidase gene (pepX) is part of the glnRA operon in Lactobacillus rhamnosus
15. Cloning and sequence analysis of the X-prolyl-dipeptidyl-aminopeptidase gene (pepX) from Lactobacillus delbrückiiei ssp. lactis DSM7290
16. Regular, nonsporing Gram-positive rods
17. A new mobile genetic element in Lactobacillus delbrueckii subsp. bulgaricus
18. Insertion sequence
19. OppA of Listeria monocytogenes, an oligopeptide-binding protein required for bacterial growth at low temperature and involved in intracellular survival
20. Sequence, assembly and analysis of pX01 and pX02
21. Oligopeptide permease is required for expression of the Bacillus thuringiensis plcR regulon and for virulence
22. DNA sequence analysis, expression, distribution, and physiological role of the Xaa-prolyldipeptidyl aminopeptidase gene from Lactobacillus helveticus CNRZ32
23. Identification of the active site serine of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis
24. Cloning and DNA sequence analysis of an X-prolyl dipeptidyl aminopeptidase gene from Lactococcus lactis subsp. lactis NCDO763
25. An X-prolyl dipeptidyl aminopeptidase (pepX) gene from Lactobacillus helveticus
26. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
27. The neighbor-joining method: A new method for reconstructing phylogenetic trees