Secretory production of recombinant human C-reactive protein in Escherichia coli, capable of binding with phosphorylcholine, and its characterization

Biochemical and Biophysical Research Communications

Volumn 295, Issue 1, 2002, Pages 163-166

  Tanaka, Toshio ^a   Horio, Takekazu ^a   Matuo, Yuhsi ^a  

^a Oriental Yeast Co Ltd   (Japan)

Author keywords

C reactive protein; Cardiovascular disease; High sensitivity CRP assay; Kil gene; Phosphorylcholine

Indexed keywords

C REACTIVE PROTEIN; COLICIN E1; MONOMER; PHOSPHORYLCHOLINE;

AFFINITY CHROMATOGRAPHY; AMINO ACID ANALYSIS; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CARDIOVASCULAR DISEASE; CARDIOVASCULAR RISK; CONTROLLED STUDY; ESCHERICHIA COLI; GENE; IMMUNOCHEMISTRY; KIL GENE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN STRUCTURE;

BACTERIAL PROTEINS; C-REACTIVE PROTEIN; CALCIUM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; IMMUNOASSAY; MOLECULAR WEIGHT; PHOSPHORYLCHOLINE; RECOMBINANT PROTEINS; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI;

EID: 0036308536     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)00622-8     Document Type: Article

References (13)

1. Serological reactions in pneumonia with a non-protein somatic fraction of the pneumococcus
2. Localization of the phosphorylcholine-binding sites on C-reactive protein by immunoelectron microscopy
3. Three dimensional structure of human C-reactive protein
4. A new sensitive assay for the calcium-dependent binding of C-reactive protein to phosphorylcholine
5. Genomic DNA sequence for human C-reactive protein
6. Characterization of genomic and complementary DNA sequence of human C-reactive protein, and comparison with the complementary DNA sequence of serum amyloid P component
7. Clinical efficacy of an automated high-sensitivity C-reactive protein assay
8. Recombinant M-, B- and MB-type isozymes of human phosphoglyceric acid mutase: Their large scale production and preparation of polyclonal antibodies specific M- and B-type isozymes
9. One-step preparation of competent Echerichia coli: Transformation and storage of bacterial cells in the same solution
10. New ligands for human C-reactive protein: Calcium-dependent binding with epsilon-aminocaproic acid-agarose and calcium-independent binding with omega-aminohexyl-agarose
11. Measurement of protein using bicinchonic acid
12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
13. CEA-kil operon of the col-E1 plasmid