Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: Implications for biosafety of bioterrorism agents

Mayo Clinic Proceedings

Volumn 77, Issue 7, 2002, Pages 624-628

  Espy, Mark J ^a   Uhl, James R ^a   Sloan, Lynne M ^a   Rosenblatt, Jon E ^a   Cockerill, Franklin R ^a   Smith, Thomas F ^a  

^a MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; VIRUS DNA;

ARTICLE; AUTOCLAVE; BACILLUS ANTHRACIS; BIOLOGICAL WARFARE; BIOSAFETY; COLONY FORMING UNIT; CONTROLLED STUDY; GENE TARGETING; GOVERNMENT; HERPES SIMPLEX VIRUS; LABORATORY; NONHUMAN; POLYMERASE CHAIN REACTION; PRACTICE GUIDELINE; PUBLIC HEALTH SERVICE; SMALLPOX; SPOROGENESIS; STANDARDIZATION; VACCINIA VIRUS; VARICELLA ZOSTER VIRUS; VIRUS DETECTION; VIRUS INACTIVATION; VIRUS INFECTIVITY;

EID: 0036307397     PISSN: 00256196     EISSN: None     Source Type: Journal    
DOI: 10.4065/77.7.624     Document Type: Article

References (15)

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7. Detection of smallpox virus DNA by LightCycler PCR
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9. Diagnosis of varicella-zoster virus infections in the clinical laboratory by LightCycler PCR
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11. Detection of herpes simplex virus DNA in genital and dermal specimens by LightCycler PCR after extraction using IsoQuick, MagNA Pure, and BioRobot 9604 methods
12. Detection of human papillomavirus (HPV) DNA in cervical swabs by the polymerase chain reaction: An evaluation of the sensitivity of the method in patients with HPV 16-harboring cervical lesions
13. Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis
14. Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection