The isomerization of the UvrB-DNA preincision complex couples the UvrB and UvrC activities

Journal of Molecular Biology

Volumn 320, Issue 1, 2002, Pages 73-84

  Delagoutte, Emmanuelle ^a   Fuchs, Robert P P ^a   Bertrand Burggraf, Elisabeth ^a  

^a CNRS   (France)

Author keywords

Complex stability; Isomerization reaction; Protein coupling; UvrB DNA preincision complex

Indexed keywords

ABC EXCINUCLEASE; ADENOSINE TRIPHOSPHATE; SINGLE STRANDED DNA;

ARTICLE; BINDING SITE; CHEMICAL PARAMETERS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SUBSTRATE; EXCISION REPAIR; ISOMERISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION;

ESCHERICHIA COLI;

EID: 0036295581     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00401-1     Document Type: Article

References (36)

1. Identification of the different intermediates in the interaction of (A)BC excinuclease with its substrates by DNase I footprinting on two uniquely modified oligonucleotides
2. Helicase properties of the Escherichia coli UvrAB protein complex
3. Characterization of the helicase activity of the Escherichia coli UvrAB protein complex
4. Electron microscopic study of (A)BC excinuclease. DNA is sharply bent in the UvrB-DNA complex
5. The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex
6. Catalytic sites for 3′ and 5′ incision of Escherichia coli nucleotide excision repair are both located in UvrC
7. Active site of (A)BC excinuclease II. Binding, bending, and catalysis mutants of UvrB reveal a direct role in 3′ and an indirect role in 5′ incision
8. Strand opening by the UvrA(2)B complex allowsdynamic recognition of DNA damage
9. The limited strand-separating activity of the UvrAB protein complex and its role in the recognition of DNA damage
10. Formation and enzymatic properties of the UvrB DNA complex
11. Hierarchy of DNA damage recognition in Escherichia coli nucleotide excision repair
12. The effect of the DNA flanking the lesion on formation of the UvrB-DNA preincision complex. Mechanism for the UvrA-mediated loading of UvrB onto a DNA damaged site
13. UvrAB activity at a damaged DNA site: Is unpaired DNA present?
14. Nucleotide excision repair in Escherichia coli
15. Acetylaminofluorene bound to different guanines of the sequence -GGCGCC- is excised with different efficiencies by the UvrABC excision nuclease in a pattern not correlated to the potency of mutation induction
16. Sequence-dependent modulation of nucleotide excision repair: The efficiency of the incision reaction is inversely correlated with the stability of the pre-incision UvrB-DNA complex
17. The C-terminal region of the UvrB protein of Escherichia coli contains an important determinant for UvrC binding to the preincision complex but not the catalytic site for 3′-incision
18. Interaction of the UvrABC endonuclease with DNA containing a psoralen monoadduct or cross-link
19. UvrABC incision of N-methylmitomycin A-DNA monoadducts and cross-links
20. Structure and dynamics of water surrounding biomolecules
21. DNA conformation and protein binding
22. On the processivity of polymerases
23. Architecture of nucleotide excision repair complexes: DNA is wrapped by UvrB before and after damage recognition
24. Interaction of UvrA and UvrB proteins with a fluorescent single-stranded DNA
25. Crystal structure of UvrB, a DNA helicase adapted for nucleotide excision repair
26. Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork
27. A general model for nucleic acid helicases and their coupling within macromolecular machines
28. The role of ATP binding and hydrolysis by UvrB during nucleotide excision repair
29. Equilibrium studies of kinesin-nucleotide intermediates
30. Molecular motors: Structural adaptations to cellular functions
31. Interactions of bacteriophage T7 DNA primase/helicase protein with single-stranded and double-stranded DNAs
32. Amplification and purification of UvrA, UvrB and UvrC proteins of Escherichia coli
33. Construction of plasmids containing a unique acetylaminofluorene adduct located within a mutational hot spot: A new probe for frameshift mutagenesis
34. Analysis of UvrABC endonuclease reaction intermediates on cisplatin-damaged DNA using mobility shift gel electrophoresis