Intact vinculin protein is required for control of cell shape, cell mechanics, and rac-dependent lamellipodia formation

Biochemical and Biophysical Research Communications

Volumn 290, Issue 2, 2002, Pages 749-755

  Goldmann, Wolfgang H ^a   Ingber, Donald E ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Cytoskeletal mechanics; Cytoskeleton; Focal adhesion; G protein; Motility

Indexed keywords

INTEGRIN; RAC PROTEIN; VINCULIN;

ANIMAL CELL; ARTICLE; CELL MOTION; CELL SHAPE; CELL SPREADING; CONTROLLED STUDY; CYTOSKELETON; EMBRYO; LAMELLIPODIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; RIGIDITY;

EID: 0036293439     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.6243     Document Type: Article

References (36)

1. Cellular tensegrity: Defining new rules of biological design that govern the cytoskeleton
2. Cell migration: Regulation of force on extracellular-matrix-integrin complexes
3. Focal adhesions: Transmembrane junctions between the extracellular matrix and the cytoskeleton
4. Mechanotransduction across the cell surface and through the cytoskeleton
5. Convergence of integrin and growth factor receptor signaling pathways within the focal adhesion complex
6. Fibronectin and integrins in cell adhesion, signaling, and morphogenesis
7. Expression of chicken vinculin complements the adhesion-defective phenotype of a mutant mouse F9 embryonal carcinoma cell
8. Motility of vinculin-deficient F9 embryonic carcinoma cells analyzed by video, laser confocal, and reflection interference contrast microscopy
9. Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion
10. Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton
11. Rescue of the mutant phenotype by reexpression of full-length vinculin in null F9 cells: Effects on cell locomotion by domain deleted vinculin
12. Differences in elasticity of vinculin-deficient F9 cells measured by magnetometry and atomic force microscopy
13. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
14. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
15. The assembly of integrin adhesion complexes requires both extracellular matrix and intracellular rho/rac GTPases
16. Rho, rac, and cdc42 regulate the assembly of multi-molecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
17. High resolution detection of mechanical forces exerted by locomoting fibroblasts on the substrate
18. An intramolecular association between the head and tail domains of vinculin modulates talin binding
19. F-actin binding site masked by the intramolecular association of vinculin head and tail domains
20. Actin activates a cryptic dimerization potential of the vinculin tail domain
21. Vinculin: A cytoskeletal target of the transforming protein of Rous sarcoma virus
22. Translocation of pp60c-src to the cytoskeleton during platelet aggregation
23. Cell spreading on extracellular matrix proteins induces tyrosine phosphorylation of tensin
24. Subtypes of protein kinase C in isolated nerve growth cones: Only type II is associated with the membrane skeleton from growth cones
25. Differences in F9 and 5.51 cell elasticity determined by cell poking and atomic force microscopy
26. Scanning force microscopy of cells and membrane proteins
27. From molecules to cells - Imaging soft samples with the AFM
28. Relative microelastic mapping of living cells by atomic force microscopy
29. Measuring the viscoelastic properties of human platelets with the atomic force microscope
30. Purification of recombinant rho/rac/G25K from E. coli
31. Microinjection of Rho and Rac into quiescent Swiss 3T3 cells
32. Mammalian cell microinjection assay to study the function of Rac and Rho
33. Vinculin knockout results in multiple organ defects during embryonic development
34. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
35. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
36. Association of p60src with Triton X-100-resistant cellular structure correlates with morphological transformation