Bacillus subtilis inorganic pyrophosphatase: The C-terminal signature sequence is essential for enzyme activity and conformational integrity

Biochemical and Biophysical Research Communications

Volumn 290, Issue 2, 2002, Pages 806-812

  Konopka, Monika A ^a   White, Scott A ^a   Young, Tom W ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Bacillus subtilis; C terminal truncation; Inorganic pyrophosphatase; Signature sequence; Site directed mutagenesis

Indexed keywords

ALANINE; INORGANIC PYROPHOSPHATASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME INACTIVATION; ENZYME STABILITY; ENZYME STRUCTURE; GENE MUTATION; GENETIC CONSERVATION; MOLECULAR SIZE; NONHUMAN; PRIORITY JOURNAL; PROTEIN POLYMERIZATION;

BACILLUS SUBTILIS;

EID: 0036291155     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.6250     Document Type: Article

References (26)

1. Systematic function analysis of Bacillus subtilis genes
2. Bacillus subtilis ORF yybQ encodes a manganese-dependent inorganic pyrophosphatase with distinctive properties: The first of a new class of soluble pyrophos-phatase?
3. Cloning and expression of a unique inorganic pyrophosphatase from Bacillus subtilis: Evidence for a new family of enzymes
4. Evolutionary aspects of inorganic pyrophosphatase
5. Crystal structure of Streptococcus mutans pyrophosphatase: A new fold for an old mechanism
6. The "open" and "closed" structures of the type-C inorganic pyrophosphatases from Bacillus subtilis and Streptococcus gordonii
7. Quaternary structure and metal ion requirement of family II pyrophosphatases from Bacillus subtilis, Streptococcus gordoniii and Streptococcus mutans
8. 2+ ions against fluoride inhibition
9. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
10. pH-regulated expression of the acid and alkaline extra-cellular proteases of Yarrowia lipolityca
11. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
12. Purification, properties, and multiple forms of a manganese-activated inorganic pyrophos-phatase from Bacillus subtilis
13. JPred: A consensus secondary structure prediction server
14. A revised mechanism for the alkaline phosphatase reaction involving three metal ions
15. Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum
16. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1
17. The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403
18. Complete genome sequence of a virulent isolate of Streptococcus pneumoniae
19. Complete genome sequence of an M1 strain of Streptococcus pyogenes
20. Whole genome sequencing of meticillin-resistant Staphylococcus aureus
21. Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima
22. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae
23. Comperative genomics of Listeria species
24. Using CLUSTAL for multiple sequence alignments