High pressure NMR reveals that apomyoglobin is an equilibrium mixture from the native to the unfolded

Journal of Molecular Biology

Volumn 320, Issue 2, 2002, Pages 311-319

  Kitahara, Ryo ^a   Yamada, Hiroaki ^a   Akasaka, Kazuyuki ^b,c   Wright, Peter E ^d  

^a KOBE UNIVERSITY   (Japan)

^b KINKI UNIVERSITY   (Japan)

^c RIKEN HARIMA INSTITUTE   (Japan)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Apomyoglobin; High pressure NMR; Local unfolding; Molten globule; Volume theorem

Indexed keywords

APOMYOGLOBIN; HYDROGEN; NITROGEN 15;

ARTICLE; CONFORMATIONAL TRANSITION; HYDRATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PRESSURE; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE; TEMPERATURE; THERMODYNAMICS;

CETACEA; PHYSETER CATODON;

EID: 0036291143     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00449-7     Document Type: Article

References (50)

1. Is apomyoglobin a molten globule? Structural characterization by NMR
2. Structural and dynamic characterization of partially folded states of apomyoglobin and implications for protein folding
3. The native state of apomyoglobin described by proton NMR spectroscopy: The A-B-G-H interface of wild-type sperm whale apomyoglobin
4. Structural characterization of a partly folded apomyoglobin intermediate
5. Three-state analysis of sperm whale apomyoglobin folding
6. Structural characterization of the molten globule and native states of apomyoglobin by solution X-ray scattering
7. Populating the equilibrium molten globule state of apomyoglobin under conditions suitable for structural characterization by NMR
8. Equilibrium NMR studies in unfolded and partly folded proteins
9. Formation of a molten globule intermediate early in the kinetic folding pathway of apomyoglobin
10. The radius of gyration of an apomyoglobin folding intermediate
11. Native and non-native secondary structure and dynamics in the pH 4 intermediate of apomyoglobin
12. Pressure-induced perturbation of apomyoglobin structure: Fluorescence studies on native and acidic compact forms
13. Determination of the volume changes for pressure-induced transitions of apomyoglobin between the native, molten globule, and unfolded states
14. High-pressure denaturation of apomyogobin
15. Pressure effect on the conformational fluctuation of apomyoglobin in the native state
16. On-line cell high pressure nuclear magnetic resonance technique: Application to protein studies
17. Pressure-induced local unfolding of the Ras-binding domain of RalGEF
18. High pressure NMR reveals a variety of fluctuating conformers in β-lactoglobulin
19. High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase
20. Two-folded conformers of ubiquitin revealed by high pressure NMR
21. Pressure-resisting cell for high-pressure, high-resolution nuclear magnetic resonance measurements at very high magnetic fields
22. Thermodynamic study of apomyoglobin structure
23. Protein folding and association insights from the interfacial and thermodynamic properties of hydrocarbons
24. Conformational properties of native sperm whale apomyoglobin in solution
25. Local stability and dynamics of apocytochrome b562 examined by the dependence of hydrogen exchange on hydrostatic pressure
26. α-Lactalbumin forms a compact molten globule in the absence of disulfide bonds
27. 1H NMR
28. Molten-globule conformation of arc repressor monomers determined by high-pressure phase diagram of Staphylococcal nuclease
29. Structural characterization of the pressure-denatured state and unfolding/refolding kinetics of Staphylococcal nuclease by synchrotron small-angle X-ray scattering and Fourier-transform infrared spectroscopy
30. Protein structure and dynamics at high pressure
31. Volume, expansivity and isothermal compressibility changes associated with temperature and pressure unfolding of Staphylococcal nuclease
32. Molten globule of human α-lactalbumin: Hydration, density, and compressibility of the interior
33. Probing the contribution of internal cavities to the volume change of protein unfolding under pressure
34. Filling a cavity dramatically increases pressure stability of the c-Myb R2 domain
35. Effect of H helix destabilizing mutations on the kinetic and equilibrium folding of apomyoglobin
36. Molten globule-like state of cytochrome c under conditions simulating those near the membrane surface
37. The methanol-induced globular and expanded denatured states of cytochrome c: A study by CD, fluorescence, NMR and small-angle X-ray scattering
38. The molten globule state is involved in the translocation of proteins across membranes?
39. Retinol-binding protein is in the molten globule state at low pH
40. Cheperonin-mediated protein folding at the surface of groEL through a molten globule-like intermediate
41. Compressibility-structure relationship of globular proteins
42. Pressure dependencies of rotational, translational, and viscous friction coefficients in water-d2, acetonitrile-d3, acetonitrile, chloroform, and benzene
43. Spining glass cell for high-pressure high-resolution NMR measurements
44. Pressure-induced changes in the folded structure of lysozyme
45. High pressure NMR study of a small protein gurmarin
46. Effect of pressure on individual hydrogen bonds in proteins. Basic pancreatic trypsin inhibitor
47. Natural abundance nitrogen-15 NMR by enhanced heteronuclear spectroscopy
48. 2H
49. NMRPipe: A multidimensional spectral processing system based on UNIX pipes
50. Association of biomolecular systems via pulsed field gradient NMR self-diffusion measurements