Development of PCR-ELISA for detection and differentiation of Didymella bryoniae from related Phoma species

Plant Disease

Volumn 86, Issue 7, 2002, Pages 710-716

  Somai, B M ^a   Keinath, A P ^a   Dean, R A ^b  

^a CLEMSON UNIVERSITY   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BIOASSAY; BIODIVERSITY; ELECTROPHORESIS; FLUORESCENCE;

POLYMERASE CHAIN REACTION;

PLANTS (BOTANY);

ARMORACIA RUSTICANA; DIDYMELLA; DIDYMELLA BRYONIAE; FUNGI; PHOMA;

EID: 0036286116     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS.2002.86.7.710     Document Type: Article

References (40)

1. Morphology and variability of the cucurbit black rot fungus
2. Physiology and pathogenicity of the cucurbit black-rot fungus
3. Anaplasma marginale: Detection of carrier cattle by PCR-ELISA
4. Rapid and sensitive colorimetric detection of Xanthomonas axonopodis pv. citri by immunocapture and a nested polymerase chain reaction assay
5. International rules for seed testing
6. Ascochyta blight of cucurbits caused by Ascochyta phaseolorum
7. Spread of Didymella bryoniae from contaminated watermelon seed and transplants in greenhouse and field environments
8. Morphological, pathological and genetic differentiation of Didymella bryoniae and Phoma spp. isolated from cucurbits
9. Colorimetric detection of specific DNA segments amplified by polymerase chain reactions
10. First report of gummy stem blight, caused by Didymella bryoniae, on watermelon transplants in California
11. Detection and location of seed-borne inoculum of Didymella bryoniae and its transmission in seedlings of cucumber and pumpkin
12. A rapid, high yield mini-prep method for isolation of total genomic DNA from fungi
13. An enzyme-linked immunosorbent assay to detect PCR products of the rfbS gene from serogroup D Salmonellae: A rapid screening prototype
14. Toxin production by Alternaria solani and its related phytotoxicity to tomato breeding lines
15. Detection of Coxiella burnetii in cow's milk by PCR-enzyme-linked immunosorbent assay combined with a novel sample preparation method
16. Enzyme-linked immunosorbent assay of viruses infecting forage legumes
17. Single-tube, nested PCR for the diagnosis of Polymyxa betae infection in sugar beet roots and colorimetric analysis of amplified products
18. AC-70154, a gummy stem blight resistant muskmelon breeding line
19. AU-Jubilant and AU-Producer watermelons
20. A terminating reagent for the peroxidase-labeled enzyme immunoassay
21. Immunoenzymatic detection of PCR products for the identification of phytoplasmas in plants
22. Comparison of chromogens for the determination of horseradish peroxidase as a marker in enzyme immunoassay
23. Recovery of genomic DNA from a fungus (Sclerotinia homoeocarpa) with high polysaccharide content
24. A comparison of three potyviruses by direct hybridization analysis
25. Simple method for high sensitivity chemiluminescence ELISA using conventional laboratory equipment
26. Detection and quantitation of hepatitis C virus RNA in serum using the polymerase chain reaction and a colorimetric enzymatic detection system
27. Breeding for disease resistance in cucurbits
28. Gummy stem blight
29. Internal transcribed spacer region 1 and 2 and random amplified polymorphic DNA analysis of Didymella bryoniae and related Phoma species isolated from cucurbits
30. PCR identification and differentiation of Didymella bryoniae and Phoma species
31. Crop losses to 14 diseases of cucumber in North Carolina from 1983-1988
32. Eight isolates of Didymella bryoniae from geographically diverse areas exhibit variation in virulence but no isolate by cultivar interaction on Cucumis sativus
33. Control of foliar diseases of cucumber with resistant cultivars and fungicides
34. Single-step protocol for preparation of plant tissue for analysis by PCR
35. Simple colorimetric microtiter plate hybridization assay for detection of amplified Mycobactertium leprae DNA
36. Factors influencing external fruit rot of cucumber caused by Didymella bryoniae
37. Mycosphaerella black rot of cucurbits