Use of degenerate primers and touchdown PCR for construction of cDNA libraries

BioTechniques

Volumn 32, Issue 6, 2002, Pages 1404-1411

  Fietto, Juliana L R ^a   DeMarco, Ricardo ^a   Verjovski Almeida, Sergio ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENES; LIBRARIES; RNA;

DEGENERATE PRIMERS;

BIOTECHNOLOGY;

COMPLEMENTARY DNA; MESSENGER RNA; SODIUM CHLORIDE; PRIMER DNA; SOLVENT;

ARTICLE; DNA LIBRARY; EXPRESSED SEQUENCE TAG; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHISTOSOMA MANSONI; TEMPERATURE; UNINDEXED SEQUENCE; ANIMAL; GENE LIBRARY; GENETICS; METHODOLOGY;

SCHISTOSOMA; SCHISTOSOMA MANSONI;

ANIMALS; DNA PRIMERS; DNA, COMPLEMENTARY; EXPRESSED SEQUENCE TAGS; GENE LIBRARY; POLYMERASE CHAIN REACTION; SCHISTOSOMA MANSONI; SOLVENTS; TEMPERATURE;

EID: 0036280961     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02326rr01     Document Type: Article

References (15)

1. The human adult skeletal muscle transcriptional profile reconstructed by a novel computational approach
2. The contribution of 700,000 "ORF sequence tags" to the definition of the human transcriptome
3. Shotgun sequencing of the human transcriptome with ORF expressed sequence tags
4. Minilibraries constructed from cDNA generated by arbitrarily primed RT-PCR: An alternative to normalized libraries for the generation of ESTs from nanogram quantities of mRNA
5. SR2 elements, non-long terminal repeat retrotransposons of the RTE-1 lineage from the human blood fluke Schistosoma mansoni
6. Increased efficiency of arbitrarily primed PCR by prolonged ramp times
7. Multiplex PCR: Critical parameters and step-by-step protocol
8. Initial sequencing and analysis of the human genome
9. Construction and characterization of a Schistosoma mansoni bacterial artificial chromosome library
10. Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences
11. The genome of Schistosoma mansoni: Isolation of DNA, its size, bases, and repetitive sequences
12. Polymorphic repeated DNA element in the genome of Schistosoma mansoni
13. Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni. Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase
14. The sequence of the human genome
15. Arbitrarily primed PCR fingerprinting of RNA