One-step, highly efficient site-directed mutagenesis by toxic protein selection

BioTechniques

Volumn 32, Issue 6, 2002, Pages 1266-1270

  Xu, W ^a   Zhang, Y ^a   Yeh, L Y ^a   Ruprecht, C R ^a   Wong Staal, F ^a   McFadden, B A ^a   Reddy, T R ^a   Ruprecht, Ruth M ^a  

^a DANA FARBER CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CLONING; ESCHERICHIA COLI; GENES; PROTEINS;

PRIMERS;

MUTAGENESIS;

PLASMID DNA; PRIMER DNA; BACTERIAL PROTEIN; BACTERIAL TOXIN; CCDB PROTEIN, PLASMID F; DNA BINDING PROTEIN; GLYCOPROTEIN GP 160; KHDRBS1 PROTEIN, HUMAN; RNA BINDING PROTEIN; SIGNAL TRANSDUCING ADAPTOR PROTEIN;

ANALYTIC METHOD; ARTICLE; FRAMESHIFT MUTATION; GENE DELETION; GENE EXPRESSION; GENE INSERTION; MOLECULAR CLONING; NONHUMAN; PRODUCTIVITY; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; CELL LINE; ESCHERICHIA COLI; GENETICS; HUMAN; PLASMID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; INSERTION SEQUENCES;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL LINE; DNA-BINDING PROTEINS; ESCHERICHIA COLI; HIV ENVELOPE PROTEIN GP160; HUMANS; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; RNA-BINDING PROTEINS;

EID: 0036277710     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02326st01     Document Type: Article

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