Primer design for PCR and sequencing in high-throughput analysis of SNPs

BioTechniques

Volumn 32, Issue 6 SUPPL., 2002, Pages

  Vieux, Ellen E ^a   Kwok, Pui Yan ^a   Miller, Raymond D ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; COMPUTER SOFTWARE; DATABASE SYSTEMS; DNA SEQUENCES; GENES;

PRIMERS;

GENETIC ENGINEERING;

PRIMER DNA;

ALLELE; ARTICLE; COMPUTER PROGRAM; DATA BASE; DNA SEQUENCE; GENE FREQUENCY; GENETIC ANALYSIS; POLYMERASE CHAIN REACTION; SINGLE NUCLEOTIDE POLYMORPHISM; EQUIPMENT DESIGN; GENOME; HUMAN; METHODOLOGY; NUCLEIC ACID DATABASE; NUCLEOTIDE SEQUENCE; QUALITY CONTROL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

DATABASES, NUCLEIC ACID; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; EQUIPMENT DESIGN; GENE FREQUENCY; GENOME, HUMAN; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; QUALITY CONTROL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA;

EID: 0036276987     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/jun0202     Document Type: Article

References (10)

1. Statistical refinement of primer design parameters
2. Universal SNP genotyping assay with fluorescence polarization detection
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4. Single-nucleotidc polymorphisms in the public domain: How useful are they?
5. A web-based tool to retrieve human genome polymorphisms from public databases
6. Primer3 on the WWW for general users and for biologist programmers
7. Optimization of the annealing temperature for DNA amplification in vitro
8. dbSNP: A database of single nucleotide polymorphisms
9. Juxtaposed regions of extensive and minimal linkage disequilibrium in human Xq25 and Xq28
10. A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms