Efficient paclitaxel production using protoplasts isolated from cultured cells of Taxus cuspidata

Planta Medica

Volumn 68, Issue 5, 2002, Pages 420-424

  Aoyagi, Hideki ^a   DiCosmo, Frank ^b,c,d   Tanaka, Hideo ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

Cell culture; Cell wall; Paclitaxel; Protoplasts; Taxaceae; Taxus cuspidata

Indexed keywords

AGAROSE; CELLULASE; CITRATE POTASSIUM; PACLITAXEL;

ARTICLE; CELL CULTURE; CELL GROWTH; CELL MEMBRANE; CELL WALL; DRUG ISOLATION; DRUG SYNTHESIS; IMMOBILIZED CELL; NONHUMAN; PROTOPLAST; SUSPENSION CELL CULTURE; TAXUS;

CELL EXTRACTS; CELL MEMBRANE; CELL WALL; CELLS, CULTURED; PACLITAXEL; PROTOPLASTS; SEEDS; TAXUS;

TAXACEAE; TAXUS; TAXUS CUSPIDATA;

EID: 0036273673     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2002-32082     Document Type: Article

References (20)

1. Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia
2. First total synthesis of taxol. 1. Functionalization of the B ring
3. Total synthesis of taxol
4. Protoplast culture and paclitaxel production by Taxus yunnanensis
5. Promotion effect of alginate on chitinase production by Wasabia japonica
6. Efficient production of chitinase by Wasabia japonica protoplasts immobilized in double-layered alginate gel fibers
7. Indole alkaloids production by Catharanthus roseus protoplasts with artificial cell walls containing guluronic acid rich alginate gel
8. In plant protoplasts, spontaneous expression of defense reactions and responsiveness to exogenous elicitors are under auxin control
9. Commercially-feasible microprapagation of mountain laurel, Kalmia latifolia, by the use of shoot-tipp culture
10. Methyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures
11. Development of an apparatus for monitoring protoplast isolation from plant tissues based on both dielectric and optical methods
12. Fluorometric estimation of viable animal cells immobilized in alginate gel
13. Measurement of viable cell and protoplast concentrations with specialized fluorometer
14. Development of an optical method for monitoring protoplast formation from cultured plant cells
15. Evaluation of parameters in the isolation of viable protoplasts from cultured tabacco cells
16. Purification and characteristics of chitinase secreted by cultured Wasabia japonica cells
17. Efficient selection of cells with high taxol content from heterogeneous Taxus cell suspensions by magnetic or fluorescent antibodies
18. Immunocytochemical localization of taxol in Taxus cuspidata
19. Production of taxol and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture