SRp30c is a repressor of 3′ splice site utilization

Molecular and Cellular Biology

Volumn 22, Issue 12, 2002, Pages 4001-4010

  Simard, Martin J ^a   Chabot, Benoit ^a  

^a UNIVERSITY OF SHERBROOKE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; NUCLEAR PROTEIN; NUCLEOTIDE DERIVATIVE; PROTEIN SRP30C; RECOMBINANT PROTEIN; REPRESSOR PROTEIN; UNCLASSIFIED DRUG;

3' UNTRANSLATED REGION; AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; BINDING SITE; EXON; GENE ACTIVITY; GENE CONTROL; GENE MUTATION; INTRON; PRIORITY JOURNAL; PROTEIN RNA BINDING; REPRESSOR GENE; RNA SPLICING;

ALTERNATIVE SPLICING; BINDING SITES; EXONS; HELA CELLS; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEIN GROUP A-B; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEINS; HUMANS; INTRONS; NUCLEAR PROTEINS; PHOSPHOPROTEINS; RECOMBINANT PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA SPLICE SITES; RNA, MESSENGER; RNA-BINDING PROTEINS;

MAMMALIA;

EID: 0036261810     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.12.4001-4010.2002     Document Type: Article

References (62)

1. Finding splice sites within a wilderness of RNA
2. A highly stable duplex structure sequesters the 5′ splice site region of hnRNP A1 alternative exon 7B
3. Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization
4. Identification of a bidirectional splicing enhancer: Differential involvement of SR proteins in 5′ or 3′ splice site activation
5. Directing alternative splicing: Cast and scenarios
6. Synthesis and purification of RNA substrates
7. An intron element modulating 5′ splice site selection in the hnRNP A1 pre-mRNA interacts with hnRNP A1
8. Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro
9. The polypyrimidine tract binding protein binds upstream of neural cell-specific c-src exon N1 to repress the splicing of the intron downstream
10. Multisite RNA binding and release of polypyrimidine tract binding protein during the regulation of c-src neural-specific splicing
11. RNA secondary structure repression of a muscle-specific exon in HeLa cell nuclear extracts
12. The U1 small nuclear ribonucleoprotein/5′ splice site interaction affects U2AF65 binding to the downstream 3′ splice site
13. Natural base-pairing interactions between 5′ splice site and branch site sequences affect mammalian 5′ splice site selection
14. An element in the 5′ common exon of the NCAM alternative splicing unit interacts with SR proteins and modulates 5′ splice site selection
15. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
16. A short sequence within two purine-rich enhancers determines 5′ splice site specificity
17. Pathways for selection of 5′ splice sites by U1 snRNPs and SF2/ASF
18. The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A
19. Mutation of an RSV intronic element abolishes both U11/U12 snRNP binding and negative regulation of splicing
20. Smooth muscle-specific switching of alpha-tropomyosin mutually exclusive exon selection by specific inhibition of the strong default exon
21. Role of an inhibitory pyrimidine element and polypyrimidine tract binding protein in repression of a regulated alpha-tropomyosin exon
22. Distinct mechanisms of splicing regulation in vivo by the Drosophila protein Sex-lethal
23. Alternative splicing: Increasing diversity in the proteomic world
24. Sorting out the complexity of SR protein functions
25. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors
26. The role of overlapping U1 and U11 5′ splice site sequences in a negative regulator of splicing
27. Regulated splicing of the Drosophila Sexlethal male exon involves a blockage mechanism
28. Sex-lethal autoregulation requires multiple cis-acting elements upstream and downstream of the male exon and appears to depend largely on controlling the use of the male exon 5′ splice site
29. A 32-nucleotide exon-splicing enhancer regulates usage of competing 5′ splice sites in a differential internal exon
30. Association of missense and 5′-splice-site mutations in tau with the inherited dementia FTDP-17
31. Initial sequencing and analysis of the human genome
32. Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor
33. Inhibition by SR proteins of splicing of a regulated adenovirus pre-mRNA
34. Protein-protein interactions and 5′-splicesite recognition in mammalian mRNA precursors
35. The N-terminal domain that distinguishes yeast from bacterial RNase III contains a dimerization signal required for efficient double-stranded RNA cleavage
36. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding
37. Regulation of alternative 3′ splice site selection by constitutive splicing factors
38. Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins
39. Alternative splicing of pre-mRNA: Developmental consequences and mechanisms of regulation
40. Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3′-terminal exon
41. An RNA splicing enhancer-like sequence is a component of a splicing inhibitor element from Rous sarcoma virus
42. SR protein splicing factors interact with the Rous sarcoma virus negative regulator of splicing element
43. SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements
44. Retroviral splicing suppressor requires three nonconsensus uridines in a 5′ splice site-like sequence
45. Splicing factors induce cystic fibrosis transmembrane regulator exon 9 skipping through a nonevolutionary conserved intronic element
46. Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein
47. The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation
48. Disruption of splicing regulated by a CUG-binding protein in myotonic dystrophy
49. Aberrant regulation of insulin receptor alternative splicing is associated with insulin resistance in myotonic dystrophy
50. Identification and characterization of three members of the human SR family of pre-mRNA splicing factors
51. Exonic splicing enhancers contribute to the use of both 3′ and 5′ splice site usage of rat beta-tropomyosin pre-mRNA
52. Control of hnRNP A1 alternative splicing: An intron element represses use of the common 3′ splice site
53. Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins
54. Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of α-actinin mutually exclusive exons
55. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex
56. The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites
57. Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c
58. Post-transcriptional regulation: The dawn of PTB
59. The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA
60. Polypyrimidine tract binding protein antagonizes exon definition
61. Intrinsic U2AF binding is modulated by exon enhancer signals in parallel with changes in splicing activity
62. PIR1, a novel phosphatase that exhibits high affinity to RNA. Ribonucleoprotein complexes