Attenuated Salmonella enterica serovar typhi live vector with inducible chromosomal expression of the T7 RNA polymerase and its evaluation with reporter genes

Plasmid

Volumn 47, Issue 2, 2002, Pages 108-119

  Santiago Machuca, Araceli E ^a   Ruiz Pérez, Fernando ^a   Delgado Dominguez, José S ^b   Becker, Ingeborg ^b   Isibasi, Armando ^c   González Bonilla, César R ^a  

^a INSTITUTO MEXICANO DEL SEGURO SOCIAL   (Mexico)

^b SECRETARÍA DE SALUD   (Mexico)

^c HOSPITAL DE ESPECIALIDADES   (Mexico)

Author keywords

Chromosomal integration; Live carriers; Salmonella typhi CVD908; T7 promoter; Vaccines

Indexed keywords

BETA GALACTOSIDASE; LUCIFERASE; RECOMBINANT ANTIGEN; RNA POLYMERASE; VACCINE;

ARTICLE; BACTERIOPHAGE T7; DNA INTEGRATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION SYSTEM; GENE FUNCTION; GENETIC COMPLEMENTATION; MICROBIAL ATTENUATION; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PROMOTER REGION; REPORTER GENE; SALMONELLA ENTERICA;

ESCHERICHIA COLI; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHI; SALMONELLA TYPHI;

EID: 0036261195     PISSN: 0147619X     EISSN: None     Source Type: Journal    
DOI: 10.1006/plas.2001.1542     Document Type: Article

References (25)

1. Expression and immunogenicity of pertussis toxin S subunit-tetanus toxin fragment C fusions in Salmonella typhi strain CVD908
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
3. Use of the nirB promoter to direct the stable expression of heterologous antigens in Salmonella oral vaccine strains: Development of a single-dose oral tetanus vaccine
4. RNA polymerase-promoter interactions: The comings and goings of RNA polymerase
5. A murine model of intranasal immunization to assess the immunogenicity of attenuated Salmonella typhi live vector vaccines in stimulating serum antibody responses to expressed antigens
6. Optimization of plasmid maintenance in the attenuated live vector vaccine strain Salmonella typhi CVD908-htrA
7. Positive selection procedure for entrapment of insertion sequence elements in Gram-negative bacteria
8. Simultaneous expression of CFA/I and CS3 colonization factor antigens of enterotoxigenic E. coli by ΔroC ΔaroD Salmonella typhi vaccine strain CVD908
9. Expression of fragment C of tetanus toxin fused to a carboxyl-terminal fragment of diphteria toxin in Salmonella typhi CVD908 vaccine strain
10. Salmonella typhi vaccine strain CVD908 expressing the circumsporozoite protein of Plasmodium falciparum: Strain construction and safety and immunogenicity in humans
11. Immunogenicity of a Salmonella typhi CVD908 candidate vaccine strain expressing the major surface protein gp63 of Leishmania mexicana mexicana
12. Vectors with restriction site banks. IV, pJRD184, a 3793-bp plasmid vector having 43 unique cloning sites
13. Construction of genetically defined double aro mutants of Salmonella typhi
14. Expression of human immunodeficiency virus antigens in an attenuated Salmonella typhi vector vaccine
15. Recent studies of T7 RNA polymerase mechanism
16. Immunogenicity of the extracellular copper/zinc superoxide dismutase of the filarial parasite Acanthochelionema viteae delivered by a two-phase vaccine strain of Salmonella typhimurium
17. Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids
18. High level heterologous expression in E. coli using the anaerobically-activated nirB promoter
19. Use of bacteriophage T7 RNA polymerase to direct the selective high-level expression of cloned genes
20. Cytokine production patterns and lymphoproliferative responses in volunteers orally immunized with attenuated vaccine strains of Salmonella typhi
21. Cytotoxic T lymphocytes after oral immunization with attenuated vaccine strains of Salmonella typhi in humans
22. Safety of live oral Salmonella typhi vaccine strains with deletions in htrA and aroC, aroD and immune response in humans
23. Safety and immune response to attenuated Salmonella enterica serovar Typhi oral live vector vaccines expressing tetanous toxin fragment C
24. Phase II clinical trial of attenuated Salmonella enterica serovar Typhi oral live vector vaccine CVD908-htrA in U.S. volunteers
25. Lactococcus lactis: High-level expression of tetanus toxin fragment C and protection against lethal challenge