Monitoring the kinetics and performance of yeast membrane ABC transporters by diS-C3(3) fluorescence

International Journal of Biochemistry and Cell Biology

Volumn 34, Issue 8, 2002, Pages 931-937

  Gaskova D ^a   Cadek R ^a   Chaloupka, R ^a   Vacata, V ^b   Gebel, J ^b   Sigler, K ^c  

^a CHARLES UNIVERSITY   (Czech Republic)

^b UNIVERSITY OF BONN   (Germany)

^c INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

ABC pumps; Energization; Fluorimetric assay; Growth phase; Kinetic features; Yeast

Indexed keywords

ABC TRANSPORTER; FLUORESCENT DYE; GLUCOSE; MEMBRANE PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CELL GROWTH; CELL LEVEL; CONTROLLED STUDY; DRUG RESISTANCE; ENZYME INHIBITION; ENZYME SPECIFICITY; FLUORESCENCE; GENE DELETION; GENE OVEREXPRESSION; KINETICS; MEMBRANE POTENTIAL; MUTANT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; YEAST; YEAST CELL;

ATP-BINDING CASSETTE TRANSPORTERS; CARBOCYANINES; FLUORESCENT DYES; FUNGAL PROTEINS; INTRACELLULAR FLUID; KINETICS; MEMBRANE POTENTIALS; MEMBRANE PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036257213     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(02)00013-4     Document Type: Article

References (8)

1. The pleiotropic drug ABC transporters from Saccharomyces cerevisiae
2. Membrane transporters and antifungal drug resistance
3. 3(3)
4. 3(3) assay
5. 3(3) in yeast
6. ATPase and multidrug transport activities of the overexpressed yeast ABC protein Yor1p
7. +-ATPase activity in wild type and mutants of Saccharomyces cerevisiae treated by some lysosomotropic compounds
8. Fluorescence monitoring of membrane energization, membrane permeabilization and pleiotropic drug resistance in yeast