Optimal ratio of degenerate primer pairs improves specificity and sensitivity of PCR

BioTechniques

Volumn 32, Issue 5, 2002, Pages 1002-1006

  Yang, Xinhai ^a   Marchand, James E ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

ACCURACY; ARTICLE; DNA TEMPLATE; GENE AMPLIFICATION; GENE SEQUENCE; INTERMETHOD COMPARISON; MEASUREMENT; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0036249667     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02325bm05     Document Type: Article

References (6)

1. Degenerate PCR-based cloning method for Eph receptors and analysis of their expression in the developing murine central nervous system and vasculature
2. A novel multigene family may encode odorant receptors: A molecular basis for odor recognition
3. Nested PCR with three highly degenerate primers for amplification and identification of DNA from related organisms
4. Degenerate and nested PCR: A highly sensitive and specific method for detection of human papillomavirus infection in cutaneous warts
5. Extension of base mispairs by Taq DNA polymerase: Implications for single nucleotide discrimination in PCR
6. PCR with degenerate primers containing deoxyinosine fails with Pfu DNA polymerase